Harwardt Rainer, Maier Elke, Reichenbach Hans, Weckesser Jürgen, Benz Roland
Institut für Biologie II, Mikrobiologie, Albert-Ludwigs-Universität, Freiburg, Germany.
J Bacteriol. 2004 Oct;186(19):6667-70. doi: 10.1128/JB.186.19.6667-6670.2004.
Detergent extracts of cell envelopes of the gliding bacterium Herpetosiphon aurantiacus formed channels in lipid bilayers. Fast protein liquid chromatography across a HiTrap-Q cation-exchange column demonstrated that a 45-kDa protein forms the channel. The observation of a channel-forming protein suggests that Herpetosiphon aurantiacus Hp a2 has a permeability barrier on its surface.
滑行细菌橙黄赫氏菌的细胞膜洗涤剂提取物在脂质双层中形成通道。通过HiTrap-Q阳离子交换柱进行的快速蛋白质液相色谱分析表明,一种45 kDa的蛋白质形成了该通道。对一种形成通道的蛋白质的观察表明,橙黄赫氏菌Hp a2在其表面具有渗透屏障。