Flores Maria Jose, Bidnenko Vladimir, Michel Bénédicte
Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, 78352 Jouy en Josas, France.
EMBO Rep. 2004 Oct;5(10):983-8. doi: 10.1038/sj.embor.7400262. Epub 2004 Sep 17.
Replication forks arrested by inactivation of the main Escherichia coli DNA polymerase (polymerase III) are reversed by the annealing of newly synthesized leading- and lagging-strand ends. Reversed forks are reset by the action of RecBC on the DNA double-strand end, and in the absence of RecBC chromosomes are linearized by the Holliday junction resolvase RuvABC. We report here that the UvrD helicase is essential for RuvABC-dependent chromosome linearization in E. coli polymerase III mutants, whereas its partners in DNA repair (UvrA/B and MutL/S) are not. We conclude that UvrD participates in replication fork reversal in E. coli.
通过使主要的大肠杆菌DNA聚合酶（聚合酶III）失活而停滞的复制叉，会因新合成的前导链和后随链末端退火而逆转。逆转的复制叉通过RecBC对DNA双链末端的作用而重置，并且在没有RecBC的情况下，染色体被霍利迪连接体解离酶RuvABC线性化。我们在此报告，UvrD解旋酶对于大肠杆菌聚合酶III突变体中RuvABC依赖性染色体线性化至关重要，而其在DNA修复中的伙伴（UvrA/B和MutL/S）则不然。我们得出结论，UvrD参与大肠杆菌中的复制叉逆转。
