Mori Masanao, Takeshima Hideo, Kuratsu Jun-ichi
Department of Neurosurgery, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan.
Int J Mol Med. 2004 Oct;14(4):505-9.
The total removal of craniopharyngiomas can be difficult because of their tendency to adhere to surrounding vital structures. Frequently, adjacent brain tissue is infiltrated by leukocytes and cytokine(s) produced by tumor cells may play a pivotal role in tissue reaction. To elucidate the molecular mechanism(s) underlying such local inflammation, we investigated the expression of interleukin (IL)-1alpha, interleukin (IL)-6, and tumor necrosis factor (TNF)-alpha in 15 craniopharyngiomas using a combination of RT-PCR, immunoblots, and immunohistochemistry. Although RT-PCR detected the expression of IL-1alpha, IL-6, and TNF-alpha, immunoblots demonstrated remarkable protein expression only for IL-6. Immunohistochemical study found that the epithelial cell layer was strongly immunoreactive for IL-6. Tumor cell layers stained weakly or negatively for IL-1alpha and TNF-alpha. Sandwich ELISA showed that levels of IL-1alpha and TNF-alpha were significantly higher in the cyst fluid than in cerebrospinal fluid (CSF) but lower than 10-fold. As the concentration of IL-6 in cyst fluid was >50,000 times that in CSF, we suggest that IL-6 plays an important role in the inflammatory reaction that occurs in the interface between the craniopharyngioma and the brain parenchyma.
由于颅咽管瘤易于附着于周围重要结构，故完全切除颅咽管瘤可能具有一定难度。通常，相邻脑组织会被白细胞浸润，肿瘤细胞产生的细胞因子可能在组织反应中起关键作用。为了阐明这种局部炎症的分子机制，我们采用逆转录聚合酶链反应（RT-PCR）、免疫印迹法和免疫组织化学相结合的方法，对15例颅咽管瘤中白细胞介素（IL）-1α、白细胞介素（IL）-6和肿瘤坏死因子（TNF）-α的表达情况进行了研究。尽管RT-PCR检测到了IL-1α、IL-6和TNF-α的表达，但免疫印迹法仅显示IL-6有明显的蛋白表达。免疫组织化学研究发现，上皮细胞层对IL-6呈强免疫反应性。肿瘤细胞层对IL-1α和TNF-α染色较弱或呈阴性。夹心酶联免疫吸附测定（ELISA）显示，囊液中IL-1α和TNF-α的水平显著高于脑脊液（CSF），但低于10倍。由于囊液中IL-6的浓度是脑脊液中的>50,000倍，我们认为IL-6在颅咽管瘤与脑实质界面发生的炎症反应中起重要作用。
