重度眼表疾病患者自体培养口腔黏膜上皮细胞移植
Transplantation of cultivated autologous oral mucosal epithelial cells in patients with severe ocular surface disorders.
作者信息
Nakamura T, Inatomi T, Sotozono C, Amemiya T, Kanamura N, Kinoshita S
机构信息
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyo-ku, Kyoto 602-0841 Japan.
出版信息
Br J Ophthalmol. 2004 Oct;88(10):1280-4. doi: 10.1136/bjo.2003.038497.
BACKGROUND/AIMS: To determine outcomes of transplants of cultivated autologous oral epithelial cells in patients with severe ocular surface disorders.
METHODS
The eyes (n = 6) of four patients with Stevens-Johnson syndrome (three eyes) or chemical burns (three eyes) were studied. Autologous oral epithelial cells, grown for 2-3 weeks on a denuded amniotic membrane carrier in the presence of 3T3 fibroblasts, were air lifted. The resultant sheet was transplanted onto the damaged eye, and acceptance of the sheet by the corneal surface was confirmed 48 hours after surgery. The success of ocular surface reconstruction, graft survival, changes in visual acuity, and postoperative complications were assessed and the quality of the cultivated oral epithelial sheet was evaluated histologically.
RESULTS
At 48 hours after transplant, the entire corneal surface of all six eyes was free of epithelial defects indicating complete survival of the transplanted oral epithelium. Visual acuity was improved in all eyes. During follow up (mean 13.8 (SD 2.9) months), the corneal surface remained stable, although all eyes manifested mild peripheral neovascularisation.
CONCLUSIONS
Autologous oral epithelial cells grown on denuded amniotic membrane can be transplanted to treat severe ocular surface disorders.
背景/目的：确定培养的自体口腔上皮细胞移植治疗严重眼表疾病患者的效果。
方法
对4例史蒂文斯-约翰逊综合征患者（3只眼）或化学伤患者（3只眼）的6只眼睛进行研究。自体口腔上皮细胞在3T3成纤维细胞存在的情况下，在无细胞羊膜载体上培养2 - 3周后进行气提培养。将所得的上皮片移植到受损眼睛上，术后48小时确认角膜表面对上皮片的接受情况。评估眼表重建的成功率、移植物存活情况、视力变化和术后并发症，并通过组织学评估培养的口腔上皮片的质量。
结果
移植后48小时，所有6只眼睛的整个角膜表面均无上皮缺损，表明移植的口腔上皮完全存活。所有眼睛的视力均有改善。在随访期间（平均13.8（标准差2.9）个月），角膜表面保持稳定，尽管所有眼睛均出现轻度周边新生血管形成。
结论
在无细胞羊膜上培养的自体口腔上皮细胞可用于移植治疗严重眼表疾病。
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