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自体培养口腔黏膜上皮细胞移植治疗眼表疾病中口腔黏膜采集的手术技术

Surgical Technique for Oral Mucosa Harvesting in Autologous Cultivated Oral Mucosal Epithelial Cell Transplantation for Ocular Surface Disorders.

作者信息

Toshida Hiroshi

机构信息

Ophthalmology, Juntendo University Shizuoka Hospital, Shizuoka, JPN.

出版信息

Cureus. 2024 Sep 18;16(9):e69648. doi: 10.7759/cureus.69648. eCollection 2024 Sep.

DOI:10.7759/cureus.69648
PMID:39429339
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11489806/
Abstract

Ex vivo cultivated oral mucosal epithelial cell transplantation (COMET) was first introduced in Japan in June 2021. This technique is used to treat limbal stem cell deficiency (LSCD). This article provides a detailed description of one of the most critical steps in COMET, which is the harvesting of oral mucosa, along with accompanying videos. The samples harvested using this method were successfully cultured into cell sheets, which were then used in surgical procedures without complications.

摘要

2021年6月，体外培养口腔黏膜上皮细胞移植术（COMET）首次在日本引入。该技术用于治疗角膜缘干细胞缺乏症（LSCD）。本文详细介绍了COMET中最关键的步骤之一，即口腔黏膜采集，并配有相关视频。用该方法采集的样本成功培养成细胞片，随后用于外科手术，未出现并发症。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87aa/11489806/c12a36fd9af0/cureus-0016-00000069648-i01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87aa/11489806/c12a36fd9af0/cureus-0016-00000069648-i01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87aa/11489806/c12a36fd9af0/cureus-0016-00000069648-i01.jpg

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1
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本文引用的文献

1
Early Clinical Outcomes of the First Commercialized Human Autologous Ex Vivo Cultivated Oral Mucosal Epithelial Cell Transplantation for Limbal Stem Cell Deficiency: Two Case Reports and Literature Review.首例商业化的人自体体外培养口腔黏膜上皮细胞移植治疗角膜缘干细胞缺陷的早期临床结果：两例病例报告及文献复习。
Int J Mol Sci. 2023 May 18;24(10):8926. doi: 10.3390/ijms24108926.
2
Clinical Trial of Autologous Cultivated Limbal Epithelial Cell Sheet Transplantation for Patients with Limbal Stem Cell Deficiency.自体培养角膜缘上皮细胞片移植治疗角膜缘干细胞缺陷症的临床试验。
Ophthalmology. 2023 Jun;130(6):608-614. doi: 10.1016/j.ophtha.2023.01.016. Epub 2023 Feb 1.
3
Systematic review of clinical research on regenerative medicine for the cornea.
角膜再生医学的临床研究系统评价。
Jpn J Ophthalmol. 2021 Mar;65(2):169-183. doi: 10.1007/s10384-021-00821-z. Epub 2021 Feb 16.
4
Ex vivo cultivated oral mucosal epithelial cell transplantation for limbal stem cell deficiency: a review.体外培养口腔黏膜上皮细胞移植治疗角膜缘干细胞缺陷：综述。
Stem Cell Res Ther. 2020 Jul 21;11(1):301. doi: 10.1186/s13287-020-01783-8.
5
Global Consensus on Definition, Classification, Diagnosis, and Staging of Limbal Stem Cell Deficiency.角膜缘干细胞缺乏症的定义、分类、诊断及分期的全球共识
Cornea. 2019 Mar;38(3):364-375. doi: 10.1097/ICO.0000000000001820.
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Navigating Market Authorization: The Path Holoclar Took to Become the First Stem Cell Product Approved in the European Union.探索市场授权：Holoclar 成为欧盟批准的首个干细胞产品所走过的道路。
Stem Cells Transl Med. 2018 Jan;7(1):146-154. doi: 10.1002/sctm.17-0003. Epub 2017 Dec 27.
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J Vasc Surg. 2016 Apr;63(4):1044-50. doi: 10.1016/j.jvs.2014.10.017. Epub 2015 Apr 30.
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Punch and spindle-shaped biopsies for collecting oral mucosal tissue for the fabrication of transplantable autologous epithelial cell sheets.为了制作可移植的自体上皮细胞片，用打孔器和纺锤形活检器采集口腔黏膜组织。
J Biomed Mater Res A. 2012 Oct;100(10):2849-54. doi: 10.1002/jbm.a.34216. Epub 2012 May 24.
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Am J Ophthalmol. 2006 Feb;141(2):267-275. doi: 10.1016/j.ajo.2005.09.003.