Blais Alexandre, Dynlacht Brian David
Department of Pathology, MSB 504, New York University School of Medicine and NYU Cancer Institute, 550 First Avenue, New York, New York 10016, USA.
Curr Opin Genet Dev. 2004 Oct;14(5):527-32. doi: 10.1016/j.gde.2004.07.003.
Understanding the role of transcription factors in governing cell-cycle progression in mammalian cells has been hindered until recently by a relative lack of genetic and genomic approaches. New approaches that harness the power of ChIP and combine this technique with DNA microarrays and bioinformatics have identified direct, physiological targets and have significantly altered our view of the E2F transcription factor that is known to play a role in regulation of cell-cycle progression. Further, the identification of additional E2F family members and factors that function in concert with E2F have considerably expanded our picture of the genetic programs that are governed by this essential regulatory factor.
直到最近，由于相对缺乏遗传和基因组学方法，人们对转录因子在调控哺乳动物细胞周期进程中所起作用的理解一直受到阻碍。利用染色质免疫沉淀（ChIP）技术并将其与DNA微阵列和生物信息学相结合的新方法，已经确定了直接的生理靶点，并显著改变了我们对E2F转录因子的看法，已知该转录因子在细胞周期进程调控中发挥作用。此外，对其他E2F家族成员以及与E2F协同发挥作用的因子的鉴定，极大地拓展了我们对受这一关键调控因子支配的遗传程序的认识。
