Malavolta Marco, Bocci Fabio, Boselli Emanuele, Frega Natale G
Dipartimento di Scienze degli Alimenti, Università Politecnica delle Marche, via Brecce Bianche, 60131 Ancona, Italy.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Oct 25;810(2):173-86. doi: 10.1016/j.jchromb.2004.07.001.
The use of HPLC coupled on-line with a mass spectrometer is a very powerful tool in order to analyze intact PLs molecular species (PMS) without the need of derivatization, thus decreasing the risk of artifacts formation. A normal-phase HPLC-ESI-MS-MS method has been developed in order to study the human blood mononuclear cell PMS composition. This method was applied to characterize PMS from seven CF subjects and from seven age-matched healthy subjects. More than 140 phospholipid molecular species from phosphatidylethanolamine (PE), plasmalogen phosphatidylethanolamine (pPE), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylcholine (PC) and sphingomyelin (Sph) were identified and compared. Differences between the two groups were found in pPE (p16:0/22:6), pPE (p18:0/22:6), PE (16:0/20:4) and PC (16:0/18:2) which were significantly lower in CF subjects and in PC (16:0/16:1) which was significantly higher in CF subjects.
将高效液相色谱（HPLC）与质谱仪在线联用是一种非常强大的工具，可用于分析完整的磷脂分子种类（PMS），而无需衍生化，从而降低了产生假象的风险。为了研究人血单核细胞的PMS组成，已开发出一种正相HPLC-ESI-MS-MS方法。该方法用于表征7名囊性纤维化（CF）患者和7名年龄匹配的健康受试者的PMS。已鉴定并比较了来自磷脂酰乙醇胺（PE）、缩醛磷脂酰乙醇胺（pPE）、磷脂酰肌醇（PI）、磷脂酰丝氨酸（PS）、磷脂酰胆碱（PC）和鞘磷脂（Sph）的140多种磷脂分子种类。发现两组之间在pPE（p16:0/22:6）、pPE（p18:0/22:6）、PE（16:0/20:4）和PC（16:0/18:2）方面存在差异，CF患者中的这些磷脂分子种类显著较低，而在PC（16:0/16:1）方面，CF患者中的该磷脂分子种类显著较高。
