[前顶体蛋白酶，一种用于检测无精子症男性精液中生精细胞的顶体标志物]

[Proacrosin, an acrosomal marker for the detection of spermatogenic cells in ejaculates from azoospermic men].

作者信息

Mitchell V, Brabant S, Koscinski I, Hermand E, Rigot J-M

机构信息

Unité fonctionnelle de spermiologie, hôpital Calmette, RDJ-1 Ouest, bd du Pr.-Jules-Leclerq, 59037 Lille, France.

出版信息

Gynecol Obstet Fertil. 2004 Sep;32(9):779-84. doi: 10.1016/j.gyobfe.2004.06.015.

DOI:10.1016/j.gyobfe.2004.06.015

PMID:15380762

Abstract

Between January 2003 and April 2004, a prospective study was performed on ejaculates from non-obstructive azoospermic men (n = 95), for the identification of spermatogenic cells using an immunohistochemical labeling for proacrosin. 48.4% of ejaculates (46/95) displayed labeled spermatogenic cells. A 38/95 (40%) of men had testicular sperm extraction (TESE) followed by intracytoplasmic sperm injection (i.c.s.i.). Testicular spermatozoa were extracted from 21 men (52.5%). The sensitivity of detecting spermatogenic cells is 66.7% in predicting the presence of testicular spermatozoa, and its specificity is 76.5%. Compared to histopathological diagnostic testicular biopsy, the detection of spermatogenic cells using proacrosin immunohistochemical method offers a predictive parameter for successful TESE. The immunohistochemical method for proacrosin has the advantages of simplicity and low cost. It could be used to predict spermatogenesis from non-obstructive azoospermic men. Further evaluation is required with extensive results to improve sensitivity and specificity.

摘要

在2003年1月至2004年4月期间，对非梗阻性无精子症男性（n = 95）的精液进行了一项前瞻性研究，通过对前顶体蛋白酶进行免疫组织化学标记来鉴定生精细胞。48.4%的精液（46/95）显示有标记的生精细胞。38/95（40%）的男性接受了睾丸精子提取（TESE），随后进行了卵胞浆内单精子注射（ICSI）。从21名男性（52.5%）中提取到了睾丸精子。检测生精细胞在预测睾丸精子存在方面的敏感性为66.7%，特异性为76.5%。与组织病理学诊断性睾丸活检相比，使用前顶体蛋白酶免疫组织化学方法检测生精细胞为成功的TESE提供了一个预测参数。前顶体蛋白酶免疫组织化学方法具有操作简单和成本低的优点。它可用于预测非梗阻性无精子症男性的精子发生情况。需要通过广泛的结果进行进一步评估以提高敏感性和特异性。