四氯乙烯经厌氧微生物还原脱氯主要生成反式-1,2-二氯乙烯。
Anaerobic microbial reductive dechlorination of tetrachloroethene to predominately trans-1,2-dichloroethene.
作者信息
Griffin Benjamin M, Tiedje James M, Löffler Frank E
机构信息
Center for Microbial Ecology, Department of Microbiology and Molecular Genetics, and Institute for Environmental Toxicology, Michigan State University, East Lansing, Michigan 48824-1325, USA.
出版信息
Environ Sci Technol. 2004 Aug 15;38(16):4300-3. doi: 10.1021/es035439g.
While most sites and all characterized PCE and TCE dechlorinating anaerobic bacteria produce cis-DCE as the major DCE isomer, significant amounts of trans-DCE are found in the environment. We have obtained microcosms from some sites and enrichment cultures that produce more trans-DCE than cis-DCE. These cultures reductively dechlorinated PCE and TCE to trans-DCE and cis-DCE simultaneously and in a ratio of 3(+/-0.5):1 that was stable through serial transfers with a variety of electron donors and occurred in both methanogenic and nonmethanogenic enrichments. Two sediment-free, nonmethanogenic enrichment cultures produced trans-DCE at rates of up to 2.5 micromol L(-1) day(-1). Dehalococcoides populations were detected in both trans-DCE producing cultures by their 16S rRNA gene sequences, and trans-DCE was produced in the presence of ampicillin. Because trans-DCE can be the major product from PCE and TCE microbial dechlorination, high fractions of trans-DCE at chloroethene-contaminated sites are not necessarily from source contamination.
虽然大多数场所及所有已鉴定的能使PCE和TCE脱氯的厌氧细菌都将顺式二氯乙烯作为主要的二氯乙烯异构体，但环境中仍存在大量反式二氯乙烯。我们从一些场所获得了微观世界样本以及富集培养物，它们产生的反式二氯乙烯比顺式二氯乙烯更多。这些培养物能将PCE和TCE同时还原脱氯为反式二氯乙烯和顺式二氯乙烯，二者比例为3（±0.5）:1，在使用多种电子供体进行连续传代培养时该比例保持稳定，且在产甲烷和不产甲烷的富集培养物中均会出现。两种无沉积物、不产甲烷的富集培养物产生反式二氯乙烯的速率高达2.5 μmol L⁻¹ 天⁻¹。通过16S rRNA基因序列在两种产生反式二氯乙烯的培养物中均检测到了脱卤球菌属菌群，并且在氨苄青霉素存在的情况下仍能产生反式二氯乙烯。由于反式二氯乙烯可能是PCE和TCE微生物脱氯的主要产物，所以在氯乙烯污染场所中高比例的反式二氯乙烯不一定源于源污染。
Zotero 插件