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将双发酵梭菌DPH-1培养物解析为两个菌群,一个是梭菌属菌株,另一个是能进行四氯乙烯脱氯的哈氏脱硫肠状菌JH1菌株。

Resolution of culture Clostridium bifermentans DPH-1 into two populations, a Clostridium sp. and tetrachloroethene-dechlorinating Desulfitobacterium hafniense strain JH1.

作者信息

Fletcher Kelly E, Ritalahti Kirsti M, Pennell Kurt D, Takamizawa Kazuhiro, Löffler Frank E

机构信息

School of Civil and Environmental Engineering, Georgia Institute of Technology, 311 Ferst Drive, Atlanta, GA 30332-0512, USA.

出版信息

Appl Environ Microbiol. 2008 Oct;74(19):6141-3. doi: 10.1128/AEM.00994-08. Epub 2008 Aug 15.

Abstract

Clostridium bifermentans strain DPH-1 reportedly dechlorinates tetrachloroethene (PCE) to cis-1,2-dichloroethene. Cultivation-based approaches resolved the DPH-1 culture into two populations: a nondechlorinating Clostridium sp. and PCE-dechlorinating Desulfitobacterium hafniense strain JH1. Strain JH1 carries pceA, encoding a PCE reductive dehalogenase, and shares other characteristics with Desulfitobacterium hafniense strain Y51.

摘要

据报道,双发酵梭菌菌株DPH-1可将四氯乙烯(PCE)脱氯生成顺式1,2-二氯乙烯。基于培养的方法将DPH-1培养物解析为两个菌群:一个是非脱氯的梭菌属菌株,另一个是能使PCE脱氯的哈氏脱硫肠状菌菌株JH1。菌株JH1携带编码PCE还原脱卤酶的pceA,并且与哈氏脱硫肠状菌菌株Y51具有其他共同特征。

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