采用高效液相色谱法同时测定大鼠血浆、羊水和组织中的齐多夫定和拉米夫定。

Simultaneous determination of zidovudine and lamivudine from rat plasma, amniotic fluid and tissues by HPLC.

作者信息

Alnouti Yazen, White Catherine A, Bartlett Michael G

机构信息

Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, The University of Georgia, Athens, GA 30602-2352, USA.

出版信息

Biomed Chromatogr. 2004 Nov;18(9):641-7. doi: 10.1002/bmc.367.

DOI:10.1002/bmc.367

PMID:15386504

Abstract

A sensitive HPLC method has been developed and validated for the simultaneous quantification of zidovudine (AZT) and lamivudine (3TC) in rat plasma, amniotic fluid and placental and fetal tissues. Samples were processed by solid-phase extraction using C2 cartridges. Chromatography was performed using a phenyl column (5 microm, 150 x 2 mm i.d.) under a flow rate of 0.2 mL/min. The mobile phase consisted of 8% acetonitrile in 5 mM 1-heptane sulfonic acid dissolved in 30 mM ammonium formate buffer (pH 3.3). The method was validated in the range 0.25-50 microg/mL for both 3TC and AZT in the four biological matrices. Finally, the method was applied to a study involving fetal transport of co-administration of these compounds in a pregnant rat.

摘要

已开发并验证了一种灵敏的高效液相色谱法，用于同时定量大鼠血浆、羊水以及胎盘和胎儿组织中的齐多夫定（AZT）和拉米夫定（3TC）。样品采用C2小柱通过固相萃取进行处理。使用苯基柱（5微米，内径150×2毫米）在流速为0.2毫升/分钟的条件下进行色谱分析。流动相由溶解于30毫摩尔甲酸铵缓冲液（pH 3.3）中的5毫摩尔1-庚烷磺酸中的8%乙腈组成。该方法在四种生物基质中对3TC和AZT的验证范围均为0.25 - 50微克/毫升。最后，该方法应用于一项关于孕鼠同时给予这些化合物时胎儿转运情况的研究。