Alnouti Yazen, White Catherine A, Bartlett Michael G
Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, The University of Georgia, Athens, GA 30602-2352, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Apr 25;803(2):279-84. doi: 10.1016/j.jchromb.2004.01.005.
An HPLC method for the quantification of lamivudine (3TC) in rat plasma, amniotic fluid, placental and fetal tissues has been developed, validated and applied to the study of the placental transport of this drug in the pregnant rat. Placental and fetal tissues were processed using liquid-liquid extraction enhanced by salting out the sample using a saturated solution of ammonium sulfate. Plasma and amniotic fluid samples were processed by protein precipitation using 2 M perchloric acid. Reverse phase chromatography was performed using a phenyl column (5 microm, 150 mm x 2 mm i.d.) under a flow rate of 0.2 ml/min. The mobile phase consisted of 5% methanol in 20 mM dibasic phosphate buffer (pH 6). The method was validated over the range from 0.1 to 50 microg/ml for plasma and amniotic fluid and 0.2-50 microg/ml for the placental and fetal tissues.
已开发、验证了一种用于定量大鼠血浆、羊水、胎盘和胎儿组织中拉米夫定（3TC）的高效液相色谱法，并将其应用于研究该药物在妊娠大鼠体内的胎盘转运。胎盘和胎儿组织采用液-液萃取法处理，通过用硫酸铵饱和溶液盐析样品来增强萃取效果。血浆和羊水样品采用2 M高氯酸进行蛋白沉淀处理。使用苯基柱（5微米，150毫米×2毫米内径）在流速为0.2毫升/分钟的条件下进行反相色谱分析。流动相由20 mM磷酸二氢盐缓冲液（pH 6）中含5%甲醇组成。该方法在血浆和羊水0.1至50微克/毫升以及胎盘和胎儿组织0.2至50微克/毫升的范围内得到验证。
