Koort Joanna, Vandamme Peter, Schillinger Ulrich, Holzapfel Wilhelm, Björkroth Johanna
Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, University of Helsinki, 00014 Helsinki, Finland.
Laboratory of Microbiology, University of Ghent, 9000 Ghent, Belgium.
Int J Syst Evol Microbiol. 2004 Sep;54(Pt 5):1621-1626. doi: 10.1099/ijs.0.63164-0.
On the basis of phenotypic and DNA-DNA reassociation studies, strain CCUG 34545T has been considered to represent a distinct Lactobacillus curvatus subspecies, Lactobacillus curvatus subsp. melibiosus. However, in several independent studies dealing with Lactobacillus sakei and L. curvatus strains, the subspecies division of L. curvatus has been found to be controversial. The original study distinguishing the two subspecies within both L. curvatus and L. sakei also lacked 16S rRNA gene sequence analyses. Therefore, the taxonomic position of L. curvatus subsp. melibiosus CCUG 34545T was re-evaluated in a polyphasic taxonomy study that included 16S rRNA gene sequence analysis, DNA-DNA reassociation, DNA G + C content determination, numerical analysis of ribotypes and whole-cell protein patterns and the examination of some fundamental phenotypic properties. The results obtained indicate that strain CCUG 34545T and its duplicate, CCUG 41580T, are Lactobacillus sakei subsp. carnosus strains and that L. curvatus subsp. melibiosus is a later synonym of L. sakei subsp. carnosus.
基于表型和DNA-DNA重杂交研究，菌株CCUG 34545T被认为代表了弯曲乳杆菌的一个独特亚种，即蜜二糖弯曲乳杆菌亚种。然而，在几项涉及清酒乳杆菌和弯曲乳杆菌菌株的独立研究中，弯曲乳杆菌的亚种划分存在争议。最初区分弯曲乳杆菌和清酒乳杆菌两个亚种的研究也缺乏16S rRNA基因序列分析。因此，在一项多相分类学研究中重新评估了蜜二糖弯曲乳杆菌CCUG 34545T的分类地位，该研究包括16S rRNA基因序列分析、DNA-DNA重杂交、DNA G + C含量测定、核糖体分型和全细胞蛋白图谱的数值分析以及一些基本表型特性的检测。所得结果表明，菌株CCUG 34545T及其重复菌株CCUG 41580T是清酒乳杆菌肉亚种菌株，蜜二糖弯曲乳杆菌是清酒乳杆菌肉亚种的晚出同物异名。
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