Torriani S, Van Reenen G A, Klein G, Reuter G, Dellaglio F, Dicks L M
Dipartimento di Scienze, Università degli Studi del Molise, Italy.
Int J Syst Bacteriol. 1996 Oct;46(4):1158-63. doi: 10.1099/00207713-46-4-1158.
Lactobacillus curvatus and Lactobacillus sake are each genetically homogeneous species, as indicated by the high levels of DNA homology (> or = 76%) exhibited by strains of these taxa. However, the results of a numerical analysis of total soluble cell protein patterns and biochemical test data revealed that there are two phenotypic subgroups within L, curvatus and two phenotypic subgroups within L. sake. The overall randomly amplified polymorphic DNA (RAPD)-PCR band patterns obtained for the majority of L. curvatus strains corresponded well to the pattern obtained for the type strain of L. curvatus (strain DSM 20019). However, six strains of L. curvatus had different, but similar, RAPD-PCR profiles and grouped in a separate genetic cluster, which was linked to one of the clusters of L. sake strains. On the basis of these results, differences in biochemical and physiological characteristics, and total soluble cell protein profiles, we describe the subspecies L. curvatus subsp. curvatus subsp. nov. and L. curvatus subsp. melibiosus subsp. nov. for L. curvatus Abo-Elnaga and Kandler 1965 (Klein et al. 1996, emended description). Strains of L. sake grouped in two RAPD-PCR clusters, which was consistent with previous reports of phenotypic heterogeneity. Strains of Lactobacillus bavaricus, including type strain LMG 9844, clustered with the type strain of L. sake (strain NCFB 2714), indicating that these organisms belong to the same genetic group. We propose that strains of L. sake Katagiri, Kitahara, and Fukami 1934 (Klein et al. 1996, emended description) should be reclassified as members of L. sake subsp. sake subsp. nov. and L. sake subsp. carnosus subsp. nov. Strains of L. bavaricus are reclassified as members of L. sake subsp. sake, and the name L. bavaricus Stetter and Stetter 1980 is rejected.
弯曲乳杆菌和清酒乳杆菌各自都是基因同质的物种，这些分类单元的菌株所表现出的高水平DNA同源性（≥76%）表明了这一点。然而，对总可溶性细胞蛋白模式和生化测试数据的数值分析结果显示，弯曲乳杆菌内有两个表型亚群，清酒乳杆菌内也有两个表型亚群。大多数弯曲乳杆菌菌株获得的总体随机扩增多态性DNA（RAPD）-PCR条带模式与弯曲乳杆菌模式菌株（DSM 20019菌株）获得的模式非常吻合。然而，六株弯曲乳杆菌具有不同但相似的RAPD-PCR图谱，并聚集在一个单独的遗传簇中，该簇与清酒乳杆菌菌株的一个簇相连。基于这些结果、生化和生理特征的差异以及总可溶性细胞蛋白图谱，我们描述了弯曲乳杆菌亚种弯曲乳杆菌新亚种和弯曲乳杆菌亚种蜜二糖乳杆菌新亚种，用于1965年的弯曲乳杆菌Abo-Elnaga和Kandler（Klein等人，1996年，修订描述）。清酒乳杆菌菌株聚集在两个RAPD-PCR簇中，这与之前关于表型异质性的报道一致。包括模式菌株LMG 9844在内的巴伐利亚乳杆菌菌株与清酒乳杆菌模式菌株（NCFB 2714菌株）聚集在一起，表明这些生物体属于同一遗传组。我们建议将1934年的清酒乳杆菌Katagiri、Kitahara和Fukami菌株（Klein等人，1996年，修订描述）重新分类为清酒乳杆菌亚种清酒新亚种和清酒乳杆菌亚种肉生新亚种的成员。巴伐利亚乳杆菌菌株被重新分类为清酒乳杆菌亚种清酒的成员，1980年的巴伐利亚乳杆菌Stetter和Stetter名称被废弃。
