Griffith G D, Cole J R, Quensen J F, Tiedje J M
Department of Crop and Soil Sciences, Michigan State University, East Lansing 48824.
Appl Environ Microbiol. 1992 Jan;58(1):409-11. doi: 10.1128/aem.58.1.409-411.1992.
Desulfomonile tiedjei DCB-1 is a strict anaerobe capable of reductively dechlorinating meta-chlorobenzoates. To probe the mechanism of this aryl dechlorination, we incubated cell suspensions of D. tiedjei in D2O and with 2,5-dichlorobenzoate. The deuterium was incorporated into the dechlorination product exclusively at the position of dehalogenation, as shown by gas chromatography-mass spectrometry and proton magnetic resonance analyses. These results favor a model for dechlorination that should not allow proton exchange at other positions, as would be the case if partial ring reduction occurred.
脱硫单胞菌（Desulfomonile tiedjei）DCB-1是一种严格厌氧菌，能够对间氯苯甲酸进行还原脱氯。为探究这种芳基脱氯的机制，我们将脱硫单胞菌的细胞悬浮液置于重水中，并加入2,5-二氯苯甲酸进行培养。气相色谱-质谱分析和质子磁共振分析表明，氘仅在脱卤位置掺入到脱氯产物中。这些结果支持了一种脱氯模型，即该模型不允许在其他位置发生质子交换，而如果发生部分环还原则会出现这种情况。
