针对代表促甲状腺激素结合区域的合成人促甲状腺激素受体肽的兔抗体的生物学活性。
Biological activities of rabbit antibodies against synthetic human thyrotropin receptor peptides representing thyrotropin binding regions.
作者信息
Sakata S, Ogawa T, Matsui I, Manshouri T, Atassi M Z
机构信息
Third Department of Internal Medicine, Gifu University School of Medicine, Japan.
出版信息
Biochem Biophys Res Commun. 1992 Feb 14;182(3):1369-75. doi: 10.1016/0006-291x(92)91884-s.
Recently, we have shown that the thyrotropin (TSH) binding regions of human thyrotropin receptor (TSHR) reside in two areas within residues 12-44 and 308-344. Serial antisera were raised against four overlapping synthetic peptides representing these two regions of TSHR (peptides 12-30, 24-44, 308-328, and 324-344) and were investigated for their ability to stimulate or block the cultured porcine thyroid cells. In addition, serum concentrations of triiodothyronine (T3) and thyroxine (T4) in serial sera obtained from each rabbit were examined. It was shown that residues of 12-30 and 324-344 of TSHR, respectively, are the site (at least a part of the site) where stimulating (TSAb) and blocking type (TSBAb) immunoglobulins are directed.
最近，我们已经表明，人促甲状腺激素受体（TSHR）的促甲状腺激素（TSH）结合区域位于12 - 44位残基和308 - 344位残基内的两个区域。针对代表TSHR这两个区域的四个重叠合成肽（肽12 - 30、24 - 44、308 - 328和324 - 344）制备了系列抗血清，并研究了它们刺激或阻断培养的猪甲状腺细胞的能力。此外，检测了从每只兔子获得的系列血清中三碘甲状腺原氨酸（T3）和甲状腺素（T4）的血清浓度。结果表明，TSHR的12 - 30位残基和324 - 344位残基分别是刺激型（TSAb）和阻断型（TSBAb）免疫球蛋白作用的位点（至少是该位点的一部分）。
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