酸性钙调蛋白结合蛋白ACAMP - 81是与突触素I相互作用的MARCKS蛋白。

Acidic calmodulin binding protein, ACAMP-81, is MARCKS protein interacting with synapsin I.

作者信息

Mizutani A, Tokumitsu H, Hidaka H

机构信息

Department of Pharmacology, Nagoya University School of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1992 Feb 14;182(3):1395-401. doi: 10.1016/0006-291x(92)91888-w.

DOI:10.1016/0006-291x(92)91888-w

PMID:1540183

Abstract

ACAMP-81 is an acidic calmodulin binding protein with molecular mass of 81 kDa. We report partial amino acid analysis of ACAMP-81 and its interaction with synapsin I. 123 amino acids of ACAMP-81 were determined and the sequence was completely identical with that of MARCKS protein which was thought to be a substrate for calcium/phospholipid dependent protein kinase (PKC). We found ACAMP-81 bound to synapsin I with 125I-labeled ACAMP-81 overlay method. ACAMP-81 bound to the cysteine specific cleaved 51 kDa fragment derived from middle/tail region of synapsin I.

摘要

ACAMP - 81是一种分子量为81 kDa的酸性钙调蛋白结合蛋白。我们报告了ACAMP - 81的部分氨基酸分析及其与突触素I的相互作用。确定了ACAMP - 81的123个氨基酸，其序列与被认为是钙/磷脂依赖性蛋白激酶（PKC）底物的MARCKS蛋白的序列完全相同。我们用125I标记的ACAMP - 81覆盖法发现ACAMP - 81与突触素I结合。ACAMP - 81与源自突触素I中/尾部区域的半胱氨酸特异性切割的51 kDa片段结合。