Yang C, Mayau V, Godeau F, Goud B
Unité de Génétique Somatique, URA CNRS 361, INSERM U 277, Institut Pasteur, Paris, France.
Biochem Biophys Res Commun. 1992 Feb 14;182(3):1499-505. doi: 10.1016/0006-291x(92)91903-4.
Rab6 protein (rab6p) belongs to a family of ras-like GTP-binding proteins thought to be involved in the regulation of intracellular transport in mammalian cells. We have constructed a recombinant baculovirus in order to express rab6p in insect cells. We report here the characterization of four forms of this protein which are found in cytosolic and membrane fractions of infected Sf9 cells. The two major forms are a cytosolic 24 kD protein which represents the unprocessed precursor form of rab6p and a membrane-bound isoprenylated 23 kD protein which represents the processed form. Two other minor forms were also detected: a cytosolic isoprenylated 23 kD protein which may represent a pool in equilibrium with the 23 kD membrane-bound form and a 24 kD non-isoprenylated membrane-bound form which may represent an intermediate in the processing of rab6p.
Rab6蛋白（rab6p）属于一类与Ras相似的GTP结合蛋白家族，被认为参与哺乳动物细胞内的转运调节。为了在昆虫细胞中表达rab6p，我们构建了一种重组杆状病毒。我们在此报告了在感染的Sf9细胞的胞质和膜部分中发现的该蛋白的四种形式的特征。两种主要形式是一种胞质24 kD蛋白，它代表rab6p的未加工前体形式，以及一种膜结合的异戊二烯化23 kD蛋白，它代表加工后的形式。还检测到另外两种次要形式：一种胞质异戊二烯化23 kD蛋白，它可能代表与23 kD膜结合形式处于平衡状态的一个库，以及一种24 kD非异戊二烯化膜结合形式，它可能代表rab6p加工过程中的一个中间体。
