甲基绿-派洛宁；核酸选择性染色的基础。
Methyl green-pyronin; basis of selective staining of nucleic acids.
作者信息
KURNICK N B
出版信息
J Gen Physiol. 1950 Jan 20;33(3):243-64, pl. doi: 10.1085/jgp.33.3.243.
Abstract
- Methyl green stains selectively highly polymerized desoxyribonucleic acid, and fails to stain, to any significant extent, depolymerized desoxyribonucleic acid and ribonucleic acid. 2. Pyronin stains preferentially low polymers of nucleic acid. 3. Triphenylmethane dyes with two amino groups appear to share the selectivity of methyl green. Those with three amino groups are not selective. 4. A stereochemical hypothesis is offered to account for these observations.
摘要
- 甲基绿选择性地对高度聚合的脱氧核糖核酸染色，而对解聚的脱氧核糖核酸和核糖核酸基本上不染色。2. 派洛宁优先对低聚核酸染色。3. 含有两个氨基的三苯甲烷染料似乎具有与甲基绿相同的选择性。含有三个氨基的则没有选择性。4. 提出了一个立体化学假说来解释这些观察结果。
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