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甲基绿。III. 与脱氧核糖核酸的反应、化学计量关系及反应产物的性质

Methyl green. III. Reaction with desoxyribonucleic acid, stoichiometry, and behavior of the reaction product.

作者信息

KURNICK N B, FOSTER M

出版信息

J Gen Physiol. 1950 Nov;34(2):147-59. doi: 10.1085/jgp.34.2.147.

DOI:10.1085/jgp.34.2.147
PMID:14824487
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2147252/
Abstract
  1. Methyl green ("ethyl green") C. I. Number 685 was examined and found to behave identically with methyl green C. I. Number 684 (no longer available) in respect to molar extinction coefficient, effect of combination with polymerized DNA, failure to react with depolymerized DNA, and effect of pH. 2. The mass law permits the calculation of P/dye. This is found to be 13 P/dye. The same value is obtained when an excess of methyl green is caused to fade by adjusting the pH to 7.5. 3. The compound formed by methyl green with DNA has the same maximum absorption at 642.5 to 645 mmicro in the pH range 3.5-7.8, whereas the free dye fades markedly above pH 5.0.
摘要
  1. 对甲基绿（“乙基绿”）C.I.编号685进行了检测，发现其在摩尔消光系数、与聚合DNA结合的效果、与解聚DNA不发生反应以及pH值的影响方面，与已不再可得的甲基绿C.I.编号684表现完全相同。2. 质量定律允许计算P/染料。结果发现其为13P/染料。当通过将pH值调节至7.5使过量的甲基绿褪色时，也能得到相同的值。3. 在pH值3.5 - 7.8范围内，甲基绿与DNA形成的化合物在642.5至645微米处具有相同的最大吸收峰，而游离染料在pH值高于5.0时会明显褪色。

相似文献

1
Methyl green. III. Reaction with desoxyribonucleic acid, stoichiometry, and behavior of the reaction product.甲基绿。III. 与脱氧核糖核酸的反应、化学计量关系及反应产物的性质
J Gen Physiol. 1950 Nov;34(2):147-59. doi: 10.1085/jgp.34.2.147.
2
Methyl green-pyronin; stoichiometry of reaction with nucleic acids.甲基绿-派洛宁；与核酸反应的化学计量学
J Gen Physiol. 1950 Jan 20;33(3):265-74. doi: 10.1085/jgp.33.3.265.
3
Methyl green-pyronin; basis of selective staining of nucleic acids.甲基绿-派洛宁；核酸选择性染色的基础。
J Gen Physiol. 1950 Jan 20;33(3):243-64, pl. doi: 10.1085/jgp.33.3.243.
4
Colorimetric determination of DNase I activity with a DNA-methyl green substrate.使用DNA-甲基绿底物比色法测定DNase I活性。
Anal Biochem. 1994 Nov 1;222(2):351-8. doi: 10.1006/abio.1994.1502.
5
Evidence of differences in the desoxyribonucleoprotein complex of rapidly proliferating and non-dividing cells.快速增殖细胞和非分裂细胞的脱氧核糖核蛋白复合物差异的证据。
J Biophys Biochem Cytol. 1955 Nov 25;1(6):531-50. doi: 10.1083/jcb.1.6.531.
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On the nature of the deoxyribonucleic acid-methyl green reaction.论脱氧核糖核酸-甲基绿反应的本质。
J Biophys Biochem Cytol. 1958 Sep 25;4(5):663-4. doi: 10.1083/jcb.4.5.663.
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[Reaction of desoxyribonucleic acids and tobacco mosaic virus with methyl green and pyronine].[脱氧核糖核酸与烟草花叶病毒与甲基绿和派洛宁的反应]
Arch Int Physiol. 1953 Apr;61(2):270-1. doi: 10.3109/13813455309147737.
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[Affinity of methyl green for desoxyribonucleic acid].[甲基绿对脱氧核糖核酸的亲和力]
Bull Soc Chim Biol (Paris). 1951;33(7):800-5.
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[Method of methyl green pyronine staining of nucleic acids].[核酸的甲基绿派洛宁染色方法]
Arch Ital Anat Embriol. 1955;60(2):126-60.
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[A method of histochemical detection of nucleic acids with the application of ribonuclease and a mixture of methyl green rhodamine 6Zh].
Arkh Patol. 1959;21(8):77-8.

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Experientia. 1965 May 15;21(5):300-4. doi: 10.1007/BF02297043.
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本文引用的文献

1
Methyl green-pyronin; stoichiometry of reaction with nucleic acids.甲基绿-派洛宁；与核酸反应的化学计量学
J Gen Physiol. 1950 Jan 20;33(3):265-74. doi: 10.1085/jgp.33.3.265.
2
Crystalline desoxyribonuclease; digestion of thymus nucleic acid; the kinetics of the reaction.结晶脱氧核糖核酸酶；胸腺核酸的消化；反应动力学
J Gen Physiol. 1950 Mar;33(4):363-77. doi: 10.1085/jgp.33.4.363.
3
Methyl green-pyronin; basis of selective staining of nucleic acids.甲基绿-派洛宁；核酸选择性染色的基础。
J Gen Physiol. 1950 Jan 20;33(3):243-64, pl. doi: 10.1085/jgp.33.3.243.