组织培养研究；定量培养技术的标准化
Tissue culture studies; standardization of techniques for quantitative culturing.
作者信息
SIGNOROTTI B, HULL W, KIRK P L
出版信息
J Gen Physiol. 1950 Mar;33(4):315-25. doi: 10.1085/jgp.33.4.315.
Abstract
Reproducible conditions have been described for quantitative tissue culturing in which all constituents including the tissue itself are added in known quantities to the culture. The conditions for transfer, cutting, and manipulation of tissue are also described. The data show that such culturing can be performed without loss or significant alteration of the nitrogen and phosphorus content of the tissue.
摘要
已经描述了用于定量组织培养的可重复条件，即在培养中以已知量添加包括组织本身在内的所有成分。还描述了组织转移、切割和操作的条件。数据表明，这种培养可以在不损失组织中氮和磷含量或使其发生显著改变的情况下进行。
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