表皮生长因子（EGF）受体在兔角膜上皮细胞、角膜基质细胞和内皮细胞中的分布，以及转化生长因子-β1诱导的变化。

Distribution of epidermal growth factor (EGF) receptors in rabbit corneal epithelial cells, keratocytes and endothelial cells, and the changes induced by transforming growth factor-beta 1.

作者信息

Hongo M, Itoi M, Yamaguchi N, Imanishi J

机构信息

Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.

出版信息

Exp Eye Res. 1992 Jan;54(1):9-16. doi: 10.1016/0014-4835(92)90063-x.

DOI:10.1016/0014-4835(92)90063-x

PMID:1541345

Abstract

Three kinds of rabbit corneal cells (epithelial cells, keratocytes and endothelial cells) were cultured, and the growth promoting effects of epidermal growth factor (EGF) were examined in these cells. It was found that the sensitivity of the epithelial cells to EGF was the highest, that of the endothelial cells was a little lower, and that of the keratocytes was the lowest. Transforming growth factor-beta 1 (TGF-beta 1) did not influence growth of the three kinds of corneal cells. It was found that TGF-beta 1 enhanced the growth promoting effect of EGF in the keratocytes, but not in the epithelial and endothelial cells. EGF receptors in the corneal cells were labelled with [125I]EGF and analysed by Scatchard plot. In the epithelial and endothelial cells, both high and low affinity receptors were found, while the keratocytes had only low affinity receptor. In the epithelial cells, the number and the association constant of the high affinity receptors were, respectively, 2.79 x 10(4) per cell and 0.034 nM, and those of the low affinity receptors were, respectively, 13.4 x 10(4) per cell and 0.700 nM. In the endothelial cells, the number and the association constant of the high affinity receptors were 1.27 x 10(4) per cell and 0.086 nM, respectively, and those of the low affinity receptors were 8.91 x 10(4) per cell and 1.536 nM. In the keratocytes, the number of receptors and the association constant were 9.49 x 10(4) per cell and 1.535 nM, respectively. The corneal cells were treated with TGF-beta 1 for 24 hr and its influence on EGF receptors was examined. The results showed that TGF-beta 1 induced the high affinity receptors in the keratocytes, although TGF-beta 1 did not influence EGF receptors in the epithelial and endothelial cells. The number of high affinity receptors in keratocytes treated with TGF-beta 1 was 1.02 x 10(4) per cell and the association constant was 0.171 nM (this was approximately tenfold higher than that of the receptor of keratocytes not treated with TGF-beta 1).

摘要

培养了三种兔角膜细胞（上皮细胞、角膜基质细胞和内皮细胞），并检测了表皮生长因子（EGF）对这些细胞的促生长作用。结果发现，上皮细胞对EGF的敏感性最高，内皮细胞次之，角膜基质细胞最低。转化生长因子-β1（TGF-β1）对这三种角膜细胞的生长均无影响。但发现TGF-β1可增强角膜基质细胞中EGF的促生长作用，而上皮细胞和内皮细胞则无此现象。用[125I]EGF标记角膜细胞中的EGF受体，并通过Scatchard作图进行分析。在上皮细胞和内皮细胞中，均发现了高亲和力和低亲和力受体，而角膜基质细胞中只有低亲和力受体。上皮细胞中，高亲和力受体的数量和结合常数分别为每个细胞2.79×10(4)个和0.034 nM，低亲和力受体的数量和结合常数分别为每个细胞13.4×10(4)个和0.700 nM。内皮细胞中，高亲和力受体的数量和结合常数分别为每个细胞1.27×10(4)个和0.086 nM，低亲和力受体的数量和结合常数分别为每个细胞8.91×10(4)个和1.536 nM。角膜基质细胞中，受体数量和结合常数分别为每个细胞9.49×10(4)个和1.535 nM。用TGF-β1处理角膜细胞24小时，检测其对EGF受体的影响。结果显示，TGF-β1可诱导角膜基质细胞产生高亲和力受体，而对上皮细胞和内皮细胞中的EGF受体无影响。经TGF-β1处理的角膜基质细胞中，高亲和力受体的数量为每个细胞1.02×10(4)个，结合常数为0.171 nM（比未用TGF-β1处理的角膜基质细胞受体约高10倍）。