Muñoz G E, Sotomayor C P
Laboratorio de Genética Molecular, Facultad de Ciencias Básicas y Matemáticas, Universidad Católica de Valparaiso, Chile.
J Appl Bacteriol. 1992 Jan;72(1):51-6. doi: 10.1111/j.1365-2672.1992.tb04881.x.
Fluidity and composition of cell membranes during progression of Mycoplasma canadense cultures grown in a serum-free medium was assessed. The fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene at 25 degrees C of intact cells and liposomes in the exponential and stationary phases of growth was compared. A decrease in fluidity and an increase in the ratio of saturated to unsaturated fatty acids was detected in cell membranes on aging. Nevertheless, membrane density remained unaltered although the molar ratio of cholesterol to phospholipids decreased. It is proposed that the increase in lipid order is primarily due to the increase in the ratio of saturated to unsaturated membrane fatty acids, being the diminished molar ratio of cholesterol to phospholipids involved in the reduced unsaturated fatty acid uptake.
评估了在无血清培养基中生长的加拿大支原体培养物进展过程中细胞膜的流动性和组成。比较了在生长的指数期和稳定期完整细胞和脂质体中1,6 - 二苯基 - 1,3,5 - 己三烯在25摄氏度下的荧光各向异性。随着细胞老化，检测到细胞膜流动性降低，饱和脂肪酸与不饱和脂肪酸的比例增加。然而，尽管胆固醇与磷脂的摩尔比降低，但膜密度保持不变。有人提出，脂质有序性的增加主要是由于膜饱和脂肪酸与不饱和脂肪酸比例的增加，而胆固醇与磷脂摩尔比的降低参与了不饱和脂肪酸摄取的减少。
