Hogan J S, Smith K L, Todhunter D A, Schoenberger P S
Department of Dairy Science, Ohio State University, Wooster.
J Dairy Sci. 1992 Jan;75(1):78-84. doi: 10.3168/jds.S0022-0302(92)77741-8.
Efficacy of an Escherichia coli (O111:B4) J5 bacterin for preventing naturally occurring IMI and clinical mastitis was tested in a 2.5-yr field trial in a 225-cow commercial herd. Cows with odd-numbered identification were vaccinated, and cows with even-numbered identification served as unvaccinated controls for each lactation during the study. Immunizations were subcutaneous on the upper part of the rib cage just posterior to the scapula at drying off, 30 d after drying off, and at calving. Percentage of quarters infected at calving with Gram-negative bacteria did not differ between treatment groups. A total of 67% of Gram-negative bacterial IMI present at calving in control cows became clinical during the first 90 d of lactation compared with 20% in vaccinated cows. Rate of Gram-negative bacterial clinical mastitis was higher in control cows than in vaccinated cows during the first 90 d of lactation. Immunization with the E. coli J5 bacterin did not reduce level of Gram-negative bacterial IMI at calving but did reduce incidence of clinical mastitis.
在一个拥有225头奶牛的商业牛群中进行了一项为期2.5年的田间试验，以测试大肠杆菌（O111:B4）J5菌苗预防自然发生的隐性乳房炎和临床型乳房炎的效果。在研究期间，识别号为奇数的奶牛接种疫苗，识别号为偶数的奶牛作为每个泌乳期的未接种疫苗对照。免疫接种在干奶期、干奶后30天和产犊时，于肩胛后方的胸廓上部皮下进行。产犊时感染革兰氏阴性菌的乳腺区百分比在各治疗组之间没有差异。对照奶牛产犊时存在的革兰氏阴性菌隐性乳房炎中，共有67%在泌乳的前90天内发展为临床型乳房炎，而接种疫苗的奶牛这一比例为20%。在泌乳的前90天内，对照奶牛的革兰氏阴性菌临床型乳房炎发生率高于接种疫苗的奶牛。用大肠杆菌J5菌苗免疫接种并没有降低产犊时革兰氏阴性菌隐性乳房炎的水平，但确实降低了临床型乳房炎的发生率。
