Soares L R, Barcinski M A
Institute of Biophysics, Federal University of Rio de Janeiro, Brazil.
J Leukoc Biol. 1992 Mar;51(3):220-4. doi: 10.1002/jlb.51.3.220.
The present results demonstrate that macrophages from mice susceptible to infection with Leishmania mexicana amazonensis sustain a higher production of granulocyte-macrophage colony-stimulating factor (GM-CSF) throughout the in vitro infection than macrophages from a resistant strain. Resident macrophages from BALB/c and C57B1/10 mice were infected with promastigotes of L. mexicana amazonensis and the amount of biologically active GM-CSF was measured in the supernatants collected at different times of infection. Measurements were made by bone marrow and GM-CSF/interleukin-3 addicted cell proliferation. Because GM-CSF is a disease-exacerbating cytokine, its differential production by infected macrophages may be one of the mechanisms defining resistance or susceptibility to a leishmanial infection.
目前的结果表明，易受亚马逊利什曼原虫感染的小鼠巨噬细胞在整个体外感染过程中，比抗性品系的巨噬细胞能持续产生更高水平的粒细胞-巨噬细胞集落刺激因子（GM-CSF）。将来自BALB/c和C57B1/10小鼠的驻留巨噬细胞用亚马逊利什曼原虫的前鞭毛体进行感染，并在感染的不同时间收集上清液，测定其中生物活性GM-CSF的含量。通过骨髓和GM-CSF/白细胞介素-3依赖细胞增殖进行测量。由于GM-CSF是一种会加剧疾病的细胞因子，受感染巨噬细胞对其的差异产生可能是决定对利什曼原虫感染具有抗性或易感性的机制之一。
