Ho J L, Reed S G, Wick E A, Giordano M
Department of Medicine, Cornell University Medical College, New York, NY 10021.
J Infect Dis. 1990 Jul;162(1):224-30. doi: 10.1093/infdis/162.1.224.
Leishmania organisms are important pathogens, causing diseases worldwide. Standard therapies are often toxic and are not always effective. The effect of recombinant human granulocyte-macrophage and macrophage colony-stimulating factors (GM-CSF and M-CSF) on intramacrophage survival of Leishmania mexicana amazonensis (Lma) were compared with those of interferon-gamma (IFN-gamma). Macrophages previously infected with Lma were treated with or without GM-CSF and M-CSF. Compared with no cytokine treatment, treatment with GM-CSF (0.1-100 ng/ml) or M-CSF (1:3.5 X 10(6) - 1:3.5 X 10(3) dilutions) caused a significant dose-dependent reduction in intracellular parasites, 427 +/- 20 (mean +/- SE) Lma/100 macrophages. GM-CSF or M-CSF in combination with IFN-gamma resulted in more effective inhibition of intracellular parasites. Thus, the cytostatic activity appears to require interaction between cytokines, macrophages, and amastigotes. These cytokines are potential therapeutic agents for visceral leishmaniasis.
利什曼原虫是重要的病原体，在全球范围内引发疾病。标准疗法往往具有毒性，且并非总能奏效。将重组人粒细胞巨噬细胞集落刺激因子和巨噬细胞集落刺激因子（GM-CSF和M-CSF）对亚马逊利什曼原虫（Lma）在巨噬细胞内生存的影响与干扰素-γ（IFN-γ）的影响进行了比较。对先前感染Lma的巨噬细胞进行有或无GM-CSF和M-CSF的处理。与未用细胞因子处理相比，用GM-CSF（0.1 - 100 ng/ml）或M-CSF（1:3.5×10⁶ - 1:3.5×10³稀释度）处理导致细胞内寄生虫显著的剂量依赖性减少，每100个巨噬细胞中有427±20个（平均值±标准误）Lma。GM-CSF或M-CSF与IFN-γ联合使用对细胞内寄生虫的抑制更有效。因此，细胞生长抑制活性似乎需要细胞因子、巨噬细胞和无鞭毛体之间的相互作用。这些细胞因子是内脏利什曼病的潜在治疗药物。
