Montagne P, Laroche P, Cuillière M L, Varcin P, Pau B, Duheille J
Immunology Laboratory, Faculty of Medicine, Nancy, France.
J Clin Lab Anal. 1992;6(1):24-9. doi: 10.1002/jcla.1860060106.
Polyfunctional hydrophilic microspheres of 125-nm diameter can be produced by copolymerization of acrylic monomers. Purified c-reactive protein (CRP) was covalently bound to these new micropheres, and the conjugate obtained was used as reagent in a microparticle-enhanced nephelometric immunoassay for human CRP. This assay was based on the measure, with a specially designed nephelometer, of the light scattered by aggregates formed during the immunoagglutination of the conjugate with anti-CRP antiserum. Sensitive inhibition of this agglutination by free CRP (6 ng/ml) allowed CRP quantitation in highly diluted serum samples (1/500-1/2,000), excluding any interference or sample pretreatment. The CRP assay was easy to perform (no washing or phase separation), reliable (coefficients of variation ranged from 1.3% to 9.3% for within-run and between-run determinations), and accurate (mean percentage of recovery: 104%; correlation coefficients with accepted analytical methods greater than or equal to 0.97) over a large range of concentrations. The inhibition mode excluded errors in the antigen excess zone and provided total security at high concentrations.
通过丙烯酸单体的共聚反应可制备直径为125纳米的多功能亲水性微球。将纯化的C反应蛋白（CRP）共价结合到这些新型微球上，所得偶联物用作人CRP微粒增强散射免疫比浊法中的试剂。该检测基于用专门设计的比浊仪测量在偶联物与抗CRP抗血清免疫凝集过程中形成的聚集体所散射的光。游离CRP（6纳克/毫升）对这种凝集的灵敏抑制作用使得能够在高度稀释的血清样本（1/500 - 1/2000）中对CRP进行定量，无需任何干扰或样本预处理。CRP检测易于操作（无需洗涤或相分离），可靠（批内和批间测定的变异系数范围为1.3%至9.3%），并且在很宽的浓度范围内准确（平均回收率：104%；与公认分析方法的相关系数大于或等于0.97）。抑制模式排除了抗原过量区的误差，并在高浓度下提供了完全保障。
