Cliquet F, Montagne P, Cuillière M L, Varcin P, Duheille J
Immunology Laboratory, Faculty of Medicine, Nancy, France.
J Clin Lab Anal. 1992;6(4):176-81. doi: 10.1002/jcla.1860060403.
A microparticle-enhanced nephelometric immunoassay was developed for myoglobin quantitation in human serum. It uses rabbit antimyoglobin serum and hydrophilic polyacrylic microparticles covalently coated with baboon myoglobin in a competitive immunoagglutination system. The level of microparticle agglutination is assessed with a specially designed nephelometer. This sensitive (45 ng/ml of myoglobin detected in serum) and accurate (coefficients of variation from 3.0% to 8.2% in precision study and linear recovery of myoglobin in overloaded sera) immunoassay was evaluated with human sera from patients suffering from acute myocardial infarction. Myoglobin levels in patient's serum on admission appeared to be correlated with clinical and biological parameters assessed in emergency wards and later. This new, rapid, and easy microparticle-enhanced nephelometric immunoassay could thus be useful in emergency conditions for the early quantitation of serum myoglobin.
开发了一种微粒增强散射比浊免疫分析法用于定量检测人血清中的肌红蛋白。该方法在竞争性免疫凝集系统中使用兔抗肌红蛋白血清和共价包被狒狒肌红蛋白的亲水性聚丙烯酸微粒。用专门设计的散射比浊仪评估微粒凝集水平。对急性心肌梗死患者的人血清进行评估，结果显示该免疫分析法灵敏（血清中可检测到45 ng/ml的肌红蛋白）且准确（精密度研究中变异系数为3.0%至8.2%，过载血清中肌红蛋白线性回收率良好）。患者入院时血清中的肌红蛋白水平似乎与急诊病房及之后评估的临床和生物学参数相关。因此，这种新型、快速且简便的微粒增强散射比浊免疫分析法在紧急情况下对血清肌红蛋白的早期定量检测可能有用。
