Koot M, Vos A H, Keet R P, de Goede R E, Dercksen M W, Terpstra F G, Coutinho R A, Miedema F, Tersmette M
Department of Clinical Viro-Immunology, Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
AIDS. 1992 Jan;6(1):49-54. doi: 10.1097/00002030-199201000-00006.
We have previously demonstrated that detection of syncytium-inducing (SI) HIV-1 in asymptomatic seropositive individuals is associated with rapid progression to AIDS. In the present study, we sought to develop and evaluate an HIV-1 phenotyping assay for the screening of large numbers of individuals.
Efficiency of HIV-1 isolation from patient peripheral blood mononuclear cells (PBMC) was studied with donor PBMC or seven different CD4+ T-cell lines as target cells. The biological phenotype of sequential isolates from 20 long-term asymptomatic HIV-1-seropositive individuals was determined by two different assays.
Non-SI isolates, efficiently recovered by cocultivation with donor PBMC, were never isolated with T-cell lines as target cells. Direct cocultivation with MT-2 cells, but not with six other CD4+ T-cells, resulted in the efficient recovery of SI isolates. HIV-1 MT-2 tropism and SI capacity were shown to be coupled properties at the clonal level. SI isolates emerged in 10 out of 20 longitudinally-studied individuals. In these long-term infected individuals, appearance of SI isolates was associated with progression to AIDS.
Direct cocultivation of patient PBMC with the MT-2 cell line is a sensitive, specific and convenient method to detect SI isolates. The availability of an assay suitable for the screening of large groups allows further study of the value of HIV-1 biological phenotyping as a prognostic marker.
我们先前已证明，在无症状血清阳性个体中检测到诱导合胞体形成（SI）的HIV-1与快速进展至艾滋病相关。在本研究中，我们试图开发并评估一种用于筛查大量个体的HIV-1表型分析方法。
以供体外周血单个核细胞（PBMC）或七种不同的CD4+ T细胞系作为靶细胞，研究从患者PBMC中分离HIV-1的效率。通过两种不同的分析方法确定了20名长期无症状HIV-1血清阳性个体连续分离株的生物学表型。
与供体PBMC共培养能有效回收的非SI分离株，从未以T细胞系作为靶细胞分离得到。与MT-2细胞直接共培养可有效回收SI分离株，但与其他六种CD4+ T细胞共培养则不行。HIV-1对MT-2细胞的嗜性和SI能力在克隆水平上显示为相关特性。在20名纵向研究的个体中，有10名出现了SI分离株。在这些长期感染的个体中，SI分离株的出现与进展至艾滋病相关。
将患者PBMC与MT-2细胞系直接共培养是检测SI分离株的一种灵敏、特异且便捷的方法。一种适用于筛查大群体样本的分析方法的出现，使得进一步研究HIV-1生物学表型作为预后标志物的价值成为可能。
