SHERMAN W B, MENZEL A E O, SEEBOHM P M
J Exp Med. 1950 Sep;92(3):191-200. doi: 10.1084/jem.92.3.191.
Sera of fourteen rabbits injected with alum-precipitated recrystallized ovaltumin, containing 0.046 to 0.604 mg. of precipitable antibody nitrogen per ml. (average 0.299 mg.), passively sensitized human skin, while the sera of nine rabbits injected with dissolved recrystallized ovalbumin, containing from less than 0.05 to 0.420 of antibody nitrogen per ml. (average 0.176 mg. or less), were inactive in human skin. The skin-sensitizing activity of the sera bore no relation to the precipitin content. Removal of 68 to 90 per cent of the precipitin nitrogen by a single addition of antigen did not affect the activity of the sera in sensitizing human skin. Removal of all precipitable antibody nitrogen in one serum by a single addition of antigen removed the skin-sensitizing activity. The "univalent" antibody remaining after complete removal of precipitin by fractional addition of antigen showed the same activity in passive sensitization of human skin as the original serum.
给14只兔子注射明矾沉淀的重结晶卵白蛋白后采集的血清，每毫升含0.046至0.604毫克可沉淀抗体氮（平均0.299毫克），能被动致敏人体皮肤；而给9只兔子注射溶解的重结晶卵清蛋白后采集的血清，每毫升含少于0.05至0.420毫克抗体氮（平均0.176毫克或更少），对人体皮肤无活性。血清的皮肤致敏活性与沉淀素含量无关。单次加入抗原去除68%至90%的沉淀素氮并不影响血清致敏人体皮肤的活性。单次加入抗原去除一种血清中所有可沉淀抗体氮后，皮肤致敏活性消失。通过分次加入抗原完全去除沉淀素后剩余的“单价”抗体，在人体皮肤被动致敏中显示出与原始血清相同的活性。
