Richardson D R, Baker E
Department of Physiology, University of Western Australia, Nedlands.
Biochim Biophys Acta. 1992 Jan 31;1103(2):275-80. doi: 10.1016/0005-2736(92)90097-6.
The effect of ferric ammonium citrate (FAC) and desferrioxamine (DFO) on membrane iron and transferrin uptake have been investigated using SK-MEL-28 human melanoma cells which express the membrane-bound transferrin homologue, melanotransferrin, at high concentrations. Exposure of melanoma cells to DFO increased membrane non-Tf-bound Fe uptake (putative melanotransferrin Fe-binding sites), suggesting upregulation of the membrane Fe-binding component. However, exposure to FAC did not result in down-regulation. Indeed, an increase in non-Tf-bound membrane Fe was apparent. Results suggested that non-Tf-bound membrane Fe uptake occurred by two processes corresponding to the specific and non-specific mechanisms of Fe uptake from Tf described previously (Richardson, D.R. and Baker, E. (1990) Biochim. Biophys. Acta 1053, 1-12).
利用高表达膜结合转铁蛋白同源物黑素转铁蛋白的SK-MEL-28人黑素瘤细胞，研究了柠檬酸铁铵（FAC）和去铁胺（DFO）对膜铁摄取和转铁蛋白摄取的影响。黑素瘤细胞暴露于DFO会增加膜非转铁蛋白结合铁的摄取（假定的黑素转铁蛋白铁结合位点），提示膜铁结合成分上调。然而，暴露于FAC并未导致下调。实际上，非转铁蛋白结合的膜铁明显增加。结果表明，非转铁蛋白结合的膜铁摄取通过两个过程发生，这两个过程分别对应于先前描述的从转铁蛋白摄取铁的特异性和非特异性机制（Richardson, D.R.和Baker, E. (1990) Biochim. Biophys. Acta 1053, 1 - 12）。
