Furuta K, Ohno S, Gibo Y, Kiyosawa K
Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
J Gastroenterol Hepatol. 1992 Jan-Feb;7(1):22-9. doi: 10.1111/j.1440-1746.1992.tb00929.x.
The ultrastructural association between the cytoskeleton and other organelles was studied by the quick-freezing and deep-etching method in rats treated with alpha-naphthylisothiocyanate (ANIT), or phalloidin, and in rats with obstructive jaundice. Cytoplasmic filaments were classified by measuring their diameters, and actin filaments were identified by specific decoration with myosin subfragment 1 (S1). S1-positive actin filaments and S1-negative intermediate filaments (12-14 nm in diameter) were observed to form a three-dimensional network around bile canaliculi, and were more numerous than in controls, not only in phalloidin-treated rats and rats with obstructive jaundice, but also in ANIT-administered rats. In all cholestatic rats, vesicular structures were also more numerous than in controls in the pericanalicular regions, and were closely associated with the microfilaments and the intermediate filaments. Filaments of a new type were localized between the lamellae of rough-surfaced endoplasmic reticulum and mitochondria, and between the lamellae of Golgi sacs and vesicles. Other thin filaments were also observed within the network of actin filaments. These filaments were 4-6 nm in diameter on replica membranes and were never decorated with S1. They were also directly connected with the canalicular membranes. Cytoskeletal components associated with membrane-bound organelles, including these new filaments, were suggested to be involved in the localization and migration of organelles.
采用快速冷冻和深度蚀刻法，研究了用α-萘基异硫氰酸盐（ANIT）或鬼笔环肽处理的大鼠以及阻塞性黄疸大鼠中细胞骨架与其他细胞器之间的超微结构关联。通过测量细胞质细丝的直径对其进行分类，并用肌球蛋白亚片段1（S1）进行特异性标记来鉴定肌动蛋白细丝。观察到S1阳性的肌动蛋白细丝和S1阴性的中间细丝（直径12 - 14纳米）在胆小管周围形成三维网络，且不仅在经鬼笔环肽处理的大鼠和阻塞性黄疸大鼠中，在给予ANIT的大鼠中也比对照组更多。在所有胆汁淤积性大鼠中，胆小管周围区域的囊泡结构也比对照组更多，并且与微丝和中间细丝紧密相关。一种新型细丝定位于糙面内质网和线粒体的板层之间以及高尔基体囊泡和小泡的板层之间。在肌动蛋白细丝网络内也观察到其他细的细丝。这些细丝在复膜上直径为4 - 6纳米，且从未被S1标记。它们也直接与胆小管膜相连。与膜结合细胞器相关的细胞骨架成分，包括这些新细丝，被认为参与了细胞器的定位和迁移。
