Marcinka K, Musil M
Acta Virol. 1977 Jan;21(1):71-8.
Red clover mottle virus was kept in 0.1 M phosphate buffer, pH 7.0, at 4, --20 and --60 degrees C for 1 and 4 weeks and 3 months. The degree of virion disintegration was the highest at --20 degrees C and the lowest at 4 degrees C. Two cycles of freezing and thawing destroyed the virions. In frozen samples (at both --20 and --60 degrees C), the nucleoprotein sedimentation component M was disintegrated more than component B.
红三叶草斑驳病毒分别在4℃、-20℃和-60℃下，于0.1M pH 7.0的磷酸盐缓冲液中保存1周、4周和3个月。病毒粒子解体程度在-20℃时最高，在4℃时最低。两轮冻融破坏了病毒粒子。在冷冻样本中（-20℃和-60℃），核蛋白沉降成分M比成分B解体得更多。
