Mabjeesh N J, Frese M, Rauen T, Jeserich G, Kanner B I
Department of Biochemistry, Hadassah Medical School, Hebrew University, Jerusalem, Israel.
FEBS Lett. 1992 Mar 24;299(1):99-102. doi: 10.1016/0014-5793(92)80109-t.
In the central nervous system, two subtypes of sodium- and chloride-coupled GABA transporter exist. One is sensitive to ACHC, the other to beta-alanine. They are thought to be of neuronal and glial origin, respectively. GABA transport in membrane vesicles derived from astroglial cells was found to be sodium- and chloride-dependent, electrogenic and much more sensitive to beta-alanine than to ACHC. Immunoblotting with antibodies directed against a variety of sequences of the ACHC-sensitive transporter indicated that none of these epitopes was shared by the glial transporter.
在中枢神经系统中，存在两种钠和氯偶联的γ-氨基丁酸（GABA）转运体亚型。一种对ACHC敏感，另一种对β-丙氨酸敏感。它们分别被认为起源于神经元和神经胶质细胞。研究发现，源自星形胶质细胞的膜囊泡中的GABA转运是钠和氯依赖性的、生电性的，并且对β-丙氨酸的敏感性远高于对ACHC的敏感性。用针对ACHC敏感转运体各种序列的抗体进行免疫印迹分析表明，这些表位均不为神经胶质转运体所共有。
