The rate of catabolism of dATP to deoxyadenosine during the growth of different cell lines in vitro.

A method has been developed for the determination of the rate of formation of deoxyadenosine from dATP in cultured cell lines. The lowest rate was found in the T-cell-derived Molt cell line while it was about 70-fold higher in Balb c/3T3 mouse fibroblasts. In the B-cell-derived Raji cells and in the murine sarcoma cell line SEWA it had intermediary values. It is concluded that in some cell types like the 3T3-cells the catabolism of dATP to deoxyadenosine may have a significant regulatory effect on the cellular content of dATP.