携带RP4 par区域的广宿主范围非可移动稳定载体的构建。
Construction of a broad-host-range non-mobilizable stable vector carrying RP4 par-region.
作者信息
Crouzet J, Lévy-Schil S, Cauchois L, Cameron B
机构信息
Département Biotechnologies, Rhône-Poulenc Rorer S.A., Vitry sur Seine, France.
出版信息
Gene. 1992 Jan 2;110(1):105-8. doi: 10.1016/0378-1119(92)90451-t.
Plasmid pXL1635 was constructed from the already segregationally stable incP-derived pRK290. Plasmid pXL1635 should be suitable for industrial and environmental uses in Gram- bacteria since (i) it contains the par fragment from RP4 which increases its stability in Pseudomonas denitrificans, a cobalamin-producing and industrially used bacterium, and (ii) the RK2 oriT has been deleted, leading to a non-mobilizable plasmid.
质粒pXL1635是由已经具有遗传稳定性的源自IncP的pRK290构建而成。质粒pXL1635应适用于革兰氏阴性菌的工业和环境应用，因为：（i）它含有来自RP4的par片段，这增加了它在反硝化假单胞菌（一种生产钴胺素且用于工业生产的细菌）中的稳定性；（ii）RK2 oriT已被删除，从而产生了一种不可转移的质粒。
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