Babic Ana, Guérout Anne-Marie, Mazel Didier
Institut Pasteur, Unité Plasticité du Génome Bactérien, CNRS URA 2171, 25 rue du Dr Roux, 75724 Paris, France.
Res Microbiol. 2008 Sep-Oct;159(7-8):545-9. doi: 10.1016/j.resmic.2008.06.004. Epub 2008 Jun 27.
In many bacteria, gene replacement can only be achieved using RP4-based conjugative systems where exogenous DNA is delivered to the recipient on conditionally replicating plasmids carrying an oriT(RP4). In the commonly used strains SM10 and S17-1 conjugative functions are provided by an RP4 plasmid inserted in the chromosome. Those strains, besides mobilizing oriT(RP4)-carrying plasmids, also transfer their own chromosomal genes, which can be inconvenient for several reasons. We describe here a new version of an RP4-based conjugative system that allows for efficient transfer of mobilizable plasmids without the transfer of chromosomal genes, providing fully controlled and efficient exogeneous DNA delivery to non-transformable bacteria.
在许多细菌中，基因替换只能通过基于RP4的接合系统来实现，其中外源DNA借助携带oriT(RP4)的条件复制质粒传递给受体。在常用菌株SM10和S17-1中，接合功能由插入染色体的RP4质粒提供。这些菌株除了能转移携带oriT(RP4)的质粒外，还会转移自身的染色体基因，这可能会因多种原因带来不便。我们在此描述一种基于RP4的新型接合系统，该系统能够高效转移可移动质粒，而不会转移染色体基因，从而为不可转化细菌提供完全可控且高效的外源DNA传递。
