Effects of physical training, methandione and their combination on the lysosomal hydrolytic activities in dog heart.

The effects of endurance training, anabolic steroid (methandione 1.5 mg/kg p.o. daily) and their combination on the activities or aryl sulphatase, B-glucuronidase and cathepsin D, all lysosomal hydrolytic enzymes, were studied in the heart of male beagle dogs. Training caused significant increase in the B-glucuronidase activity in the right ventricular wall. Anabolic steroid as such caused no change in any enzymic activities, whereas exercise combined with steroid treatment resulted in significant increases in the activities of all enzymes studied in the right ventricular wall. The combined effect of exercise training and anabolic steroid on the lysosomal hydrolytic enzymes may reflect and mediate the previous observed degenerative effects of this combination in the heart.