Martini B, Pandey R, Ogden T E, Ryan S J
Department of Ophthalmology (Doheny Eye Institute), University of Southern California School of Medicine, Los Angeles 90033.
Invest Ophthalmol Vis Sci. 1992 Mar;33(3):516-21.
The extracellular matrix of retinal pigment epithelial (RPE) cells is an important component of the fibroelastic membranes formed in proliferative vitreoretinopathy (PVR). These membranes consist of cells intermingled with fibrillar proteins, such as elastin and collagen. Because RPE cells may be exposed to vitreous and serum-derived factors in conditions that lead to PVR, the effects of vitreous and serum on the deposition of fibrillar proteins by RPE cells were studied using radioactive labeling and indirect immunofluorescence techniques. The RPE cells, modulated by combined vitreous and serum into a fibrocytic-like appearance, deposited less fibroelastic proteins per cell. However, as a result of simultaneous cell proliferation, the total deposition of fibroelastic proteins per culture was increased. Indirect immunofluorescence studies suggest that it is type I collagen that is altered in the matrix of these modulated cell cultures. This information is important in pathologic conditions characterized by intraocular cell proliferation and fibroelastic membrane formation; these phenomena are seen in many vitreoproliferative disorders.
视网膜色素上皮（RPE）细胞的细胞外基质是增殖性玻璃体视网膜病变（PVR）中形成的纤维弹性膜的重要组成部分。这些膜由与纤维状蛋白质（如弹性蛋白和胶原蛋白）混合的细胞组成。由于在导致PVR的情况下，RPE细胞可能会暴露于玻璃体和血清来源的因子中，因此使用放射性标记和间接免疫荧光技术研究了玻璃体和血清对RPE细胞纤维状蛋白质沉积的影响。经玻璃体和血清共同作用而呈现成纤维细胞样外观的RPE细胞，每个细胞沉积的纤维弹性蛋白较少。然而，由于细胞同时增殖，每个培养物中纤维弹性蛋白的总沉积量增加。间接免疫荧光研究表明，在这些经调节的细胞培养物的基质中发生改变的是I型胶原蛋白。这一信息在以眼内细胞增殖和纤维弹性膜形成为特征的病理状况中很重要；这些现象在许多玻璃体增殖性疾病中都可见到。
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