Williams D F, Burke J M
Department of Ophthalmology, Medical College of Wisconsin, Milwaukee 53226.
Invest Ophthalmol Vis Sci. 1990 Sep;31(9):1717-23.
Intravitreal membranes from patients with proliferative vitreoretinopathy (PVR) consist partly of retinal glial (RG) and retinal pigment epithelial (RPE) cells surrounded by varying amounts of extracellular matrix (ECM). The contribution of the ECM to the growth of PVR membranes is unknown. This study was undertaken to determine if proliferation in cultured RPE and RG cells is affected by different substrates, including some ECM materials which have been identified in PVR membranes. Substrates tested included type I collagen, basement membrane Matrigel, and poly-D-lysine, as well as uncharacterized cell type-specific matrices deposited by cultured RPE and RG cells. Proliferation was quantified by 3H-thymidine incorporation and radioautography 24 hours after plating and by cell counts after 14 days in the presence of serum. Relative to uncoated culture plastic, growth of RPE cells was inhibited by Matrigel, enhanced by poly-D-lysine, and unaffected by type I collagen. In contrast, growth in RG cells was inhibited by type I collagen and unaffected by the other substrates. Analysis of the timing of DNA synthesis after plating suggested that the substrates which affected RPE growth did so by altering the fraction of cycling cells rather than the cell cycle time. For the cell-derived matrices, heterotypic matrix (matrix produced by the other retinal cell type) enhanced the growth of both RPE and RG. The results suggest that the ECM may modify the growth of cells contributing to PVR membranes. Of note is that the cell-derived matrices reciprocally stimulated growth of RG and RPE cells, cell types which may interact in PVR membranes.
增殖性玻璃体视网膜病变（PVR）患者的玻璃体内膜部分由视网膜神经胶质（RG）细胞和视网膜色素上皮（RPE）细胞组成，周围环绕着不同数量的细胞外基质（ECM）。ECM对PVR膜生长的作用尚不清楚。本研究旨在确定培养的RPE细胞和RG细胞的增殖是否受不同底物的影响，包括一些在PVR膜中已鉴定出的ECM材料。测试的底物包括I型胶原、基底膜基质胶和聚-D-赖氨酸，以及培养的RPE细胞和RG细胞沉积的未表征的细胞类型特异性基质。接种24小时后通过3H-胸腺嘧啶核苷掺入和放射自显影定量增殖，在有血清存在的情况下培养14天后通过细胞计数定量增殖。相对于未包被的培养塑料，基质胶抑制RPE细胞生长，聚-D-赖氨酸促进其生长，I型胶原对其无影响。相比之下，I型胶原抑制RG细胞生长，其他底物对其无影响。接种后DNA合成时间分析表明，影响RPE细胞生长的底物是通过改变循环细胞比例而非细胞周期时间来实现的。对于细胞衍生的基质，异型基质（由另一种视网膜细胞类型产生的基质）促进RPE细胞和RG细胞的生长。结果表明，ECM可能会改变促成PVR膜的细胞的生长。值得注意的是，细胞衍生基质相互刺激RG细胞和RPE细胞的生长，这两种细胞类型可能在PVR膜中相互作用。
