Curreli Nicoletta, Rescigno Antonio, Rinaldi Augusto, Pisu Brunella, Sollai Francesca, Sanjust Enrico
Dipartimento di Scienze e Tecnologie Biomediche, Sezione di Chimica Biologica e Biotecnologie Biochimiche, Università di Cagliari, Cittadella Universitaria, 09042 Monserrato, Cagliari, Italy.
Mycol Res. 2004 Aug;108(Pt 8):913-8. doi: 10.1017/s0953756204000632.
The toxic naphthoquinone juglone (5-hydroxy-1,4-naphthoquinone) is efficiently degraded by the ligninolytic fungus Pleurotus sajor-caju, as demonstrated by the total bleaching within 9 d of a conventional liquid culture medium supplemented with 0.6 mM juglone. The oxidative degradation involves the production of hydrogen peroxide arising from both enzymic and non-enzymic oxidation reactions, promoted by the fungus. Juglone is not directly attacked by the oxidative enzymes of the ligninolytic machinery of P. sajor-caju, such as laccase, manganese peroxidase and arylalcohol oxidase. On the other hand, this naphthoquinone is a good substrate for a reductase, which triggers an auto-oxidative process producing reactive oxygen species and leading to juglone degradation. The degradation process continues to completion by means of a direct, presumably non-catalysed reaction with hydrogen peroxide.
有毒的萘醌胡桃醌（5-羟基-1,4-萘醌）能被木质素分解真菌凤尾菇有效降解，在添加了0.6 mM胡桃醌的传统液体培养基中，9天内完全脱色即证明了这一点。氧化降解涉及真菌促进的酶促和非酶促氧化反应产生的过氧化氢。胡桃醌不会被凤尾菇木质素分解机制的氧化酶如漆酶、锰过氧化物酶和芳醇氧化酶直接攻击。另一方面，这种萘醌是一种还原酶的良好底物，该还原酶引发自氧化过程，产生活性氧并导致胡桃醌降解。降解过程通过与过氧化氢的直接（可能是非催化的）反应持续进行直至完成。
