Avraham H, Vannier E, Chi S Y, Dinarello C A, Groopman J E
Division of Hematology/Oncology, New England Deaconess Hospital, Harvard Medical School, Boston, Massachusetts 02215.
Int J Cell Cloning. 1992 Mar;10(2):70-9. doi: 10.1002/stem.5530100203.
Cytokine expression and production by human megakaryocytic cells were studied using the CMK cell line as a model for cytokine gene expression by cell line as a model for cytokine gene expression by polymerase chain reaction (PCR) and for cytokine protein synthesis by specific radioimmunoassays. CMK cells at all stages of maturation were found to constitutively express moderate mRNA levels for tumor necrosis factor (TNF-alpha), transforming growth factor beta (TGF-beta), interleukin (IL) 1 beta, and endothelial cell growth factor (ECGF) transcripts. After 6-h treatment with the phorbol ester PMA, gene expression for IL-1 alpha, granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-3, and the IL-6 receptor were increased. After 24 h of exposure to PMA, levels for most cytokines declined to baseline, except for IL-6 which appeared as a new transcript. PMA-stimulated CMK lines synthesized low levels of TNF-alpha and IL-6, and higher levels of GM-CSF, IL-1 beta, and IL-1 alpha protein. These observations suggest that cells of megakaryocytic lineage are capable of producing a repertoire of cytokines which could mediate an autocrine role as well as modulate the replication and function of other hematopoietic cells.
以CMK细胞系作为细胞因子基因表达的模型，采用聚合酶链反应（PCR）研究人巨核细胞的细胞因子表达和产生情况，并通过特异性放射免疫测定法研究细胞因子蛋白的合成。发现处于所有成熟阶段的CMK细胞组成性表达中等水平的肿瘤坏死因子（TNF-α）、转化生长因子β（TGF-β）、白细胞介素（IL）1β和内皮细胞生长因子（ECGF）转录本的mRNA。在用佛波酯PMA处理6小时后，IL-1α、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、IL-3和IL-6受体的基因表达增加。在暴露于PMA 24小时后，除了作为新转录本出现的IL-6外，大多数细胞因子的水平下降至基线。PMA刺激的CMK细胞系合成低水平的TNF-α和IL-6，以及高水平的GM-CSF、IL-1β和IL-1α蛋白。这些观察结果表明，巨核细胞系细胞能够产生一系列细胞因子，这些细胞因子可以介导自分泌作用，并调节其他造血细胞的复制和功能。
