骨髓涂片上的可染铁应如何评估？
How should stainable iron in bone marrow films be assessed?
作者信息
Hughes D A, Stuart-Smith S E, Bain B J
机构信息
Department of Academic Haematology, St Mary's Hospital, Praed Street, London W2 1NY, UK.
出版信息
J Clin Pathol. 2004 Oct;57(10):1038-40. doi: 10.1136/jcp.2003.015834.
AIMS
To identify how many particles should be examined to enable a confident assessment of the presence or absence of iron stores and the quantity of iron in a bone marrow aspirate to be made.
METHODS
One hundred and ninety consecutive bone marrow aspirate samples were stained with Perls' stain and the iron content of 10 consecutive particles was recorded. The first particle found to be positive and the particle that was most positive were also noted.
RESULTS
A minimum of seven particles must be examined to establish the absence of stainable iron. A minimum of nine particles must be reviewed to see the maximum iron stores in 100% of samples and therefore make a valid judgment of whether iron stores are reduced, normal, or increased. By these criteria, 46% of the samples tested here could not be optimally assessed for absence of iron or maximum iron stores.
CONCLUSIONS
The sensitivity of examination of bone marrow aspirates for iron stores can be optimised by increasing the number of particles reviewed to seven or more. This may require the staining of additional slides.
目的
确定应检查多少个颗粒，以便能够可靠地评估骨髓穿刺液中是否存在铁储存以及铁的含量。
方法
对190份连续的骨髓穿刺液样本进行普鲁士蓝染色，并记录连续10个颗粒的铁含量。还记录了第一个呈阳性的颗粒和最呈阳性的颗粒。
结果
必须检查至少七个颗粒才能确定不存在可染色铁。必须检查至少九个颗粒才能在100%的样本中看到最大铁储存量，从而对铁储存量是减少、正常还是增加做出有效判断。根据这些标准，此处测试的46%的样本无法针对铁缺乏或最大铁储存量进行最佳评估。
结论
通过将检查的颗粒数量增加到七个或更多，可以优化骨髓穿刺液中铁储存检查的敏感性。这可能需要对额外的玻片进行染色。
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