Nikicicz E P, Norback D H
Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison.
Mod Pathol. 1991 May;4(3):363-7.
Previous observations indicated that a colloidal silver (AgNOR) method identifies iron-containing bone marrow cells. In this study we examined AgNOR-stained bone marrow smears from 30 selected patients and compared the results with the conventional Prussian blue (PB) method for staining storage iron and sideroblasts, including ringed sideroblasts (RS). Erythroid cells were easily identified, and iron granules stained as distinct, small, round, black dots. In all cases containing sideroblasts and RS, their proportion expressed as the percentage of all normoblasts was higher with AgNOR than with PB. Pronormoblastic and basophilic sideroblasts were detected more often with AgNOR than with PB. Sideroblasts were noted in cases with no PB-stainable iron. Ringed sideroblasts were identified in a number of cases in which they were nondetectable with PB. In cases of myelodysplastic syndrome, pronormoblastic and basophilic RS were found more often than with PB. Hemosiderin deposits within the macrophages were clearly visualized as black aggregates and were seen in cases with no PB-stainable iron. There were no instances where iron or any category of sideroblasts was seen with PB only. In our sampling, the AgNOR method was more sensitive yet specific, and it produced staining results of superior quality.
先前的观察表明，一种胶体银（AgNOR）方法可识别含铁的骨髓细胞。在本研究中，我们检查了30例选定患者的AgNOR染色骨髓涂片，并将结果与传统的普鲁士蓝（PB）方法进行比较，后者用于染色储存铁和环形铁粒幼细胞（RS）等铁粒幼细胞。红系细胞易于识别，铁颗粒染成清晰、小而圆的黑色小点。在所有含有铁粒幼细胞和RS的病例中，以所有早幼红细胞的百分比表示，AgNOR检测到的它们的比例高于PB。原幼红细胞性和嗜碱性铁粒幼细胞用AgNOR检测比用PB更常见。在无PB可染铁的病例中也发现了铁粒幼细胞。在许多用PB无法检测到环形铁粒幼细胞的病例中，用AgNOR检测到了它们。在骨髓增生异常综合征病例中，原幼红细胞性和嗜碱性RS比用PB更常见。巨噬细胞内的含铁血黄素沉积清晰可见为黑色聚集体，在无PB可染铁的病例中也可见到。没有仅用PB检测到铁或任何类型铁粒幼细胞的情况。在我们的样本中，AgNOR方法更敏感且特异，并且产生的染色结果质量更高。
