Platonova T A, Evsiunina A S, Korableva N P
Prikl Biokhim Mikrobiol. 2004 Jul-Aug;40(4):488-96.
The ultrastructure of the mitochondrial apparatus of apical tuber cells of original and transgenic (defensin gene-transfected) potato have been compared in normal and ambiol-treated plants, using morphometric approaches. No qualitative or quantitative differences were found between the mitochondria of original and transgenic plants under normal conditions (control). Treatment with ambiol produced only quantitative differences (in the number of mitochondria and their volume) between the cells of original and transgenic plants. This observation has been attributed to (1) changes in the physiology and biochemistry of transgenic plants, induced by the expression of the gene of defensin (hormonal balance, functional activity of the plasmalemmata, etc.), and (2) direct effects of ambiol.
利用形态计量学方法,比较了正常条件下和经安比奥处理的原始马铃薯和转基因(防御素基因转染)马铃薯顶端块茎细胞线粒体装置的超微结构。在正常条件下(对照),原始植株和转基因植株的线粒体在定性或定量方面均未发现差异。安比奥处理仅使原始植株和转基因植株的细胞在(线粒体数量及其体积方面)产生了定量差异。这一观察结果归因于:(1)防御素基因表达诱导转基因植株生理生化发生变化(激素平衡、质膜功能活性等);(2)安比奥的直接作用。