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[用安比奥处理的原始和转基因马铃薯植株顶端块茎细胞中质体的超微形态学研究]

[Ultramorphometric study of the plastids in apical tuber cells of original and transgenic potato plants treated with ambiol].

作者信息

Platonova T A, Evsiunina A S, Belikov S V, Korableva N P

出版信息

Prikl Biokhim Mikrobiol. 2005 May-Jun;41(3):330-9.

PMID:15977795
Abstract

Ultramorphometric characteristics of plastids in cells of apical tuber meristems of original and defensin gene-transfected potato (Solanum tuberosum L.) plants, either maintained under normal conditions or subjected to treatment with the antioxidant ambiol, were compared. Under normal conditions, the tuber cells of the original and transgenic potato plants differed in neither the number nor size of the plastids. Only certain quantitative distinctions in the development of individual ultrastructural characteristics of plastids were detected. Treatment with ambiol enhanced the differentiation of the internal membrane system of plastids in the cells of original and transgenic plants, especially the tubular membrane systems. Certain differences in the responses to ambiol of cell plastids of original and transgenic plants were related to plastid sizes and development of individual intraplastid structures. The results comply with earlier data on varying responses of mitochondria of original and transgenic plants to ambiol treatment.

摘要

比较了原始马铃薯(Solanum tuberosum L.)和防御素基因转染马铃薯植株顶端块茎分生组织细胞中质体的超微形态特征,这些植株要么在正常条件下培养,要么用抗氧化剂安比奥进行处理。在正常条件下,原始马铃薯植株和转基因马铃薯植株的块茎细胞在质体数量和大小上均无差异。仅在质体个体超微结构特征的发育方面检测到某些数量上的差异。用安比奥处理增强了原始植株和转基因植株细胞中质体内膜系统的分化,尤其是管状膜系统。原始植株和转基因植株的细胞质体对安比奥的反应存在某些差异,这与质体大小和质体内个体结构的发育有关。这些结果与早期关于原始植株和转基因植株线粒体对安比奥处理反应不同的数据相符。

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