采用液相色谱 -（串联）质谱法对全血中33种苯二氮䓬类药物、代谢物及苯二氮䓬样物质进行定量分析。
Quantitative analysis of 33 benzodiazepines, metabolites and benzodiazepine-like substances in whole blood by liquid chromatography-(tandem) mass spectrometry.
作者信息
Smink B E, Brandsma J E, Dijkhuizen A, Lusthof K J, de Gier J J, Egberts A C G, Uges D R A
机构信息
Department of Toxicology, Netherlands Forensic Institute, PO Box 3110, 2280 GC Rijswijk, The Netherlands.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Nov 5;811(1):13-20. doi: 10.1016/j.jchromb.2004.03.079.
A quantitative method using high-performance liquid chromatography-mass spectrometry (LC-MS, ion trap) after matrix supported liquid-liquid extraction is described for the simultaneous determination in whole blood of 33 benzodiazepines including metabolites and benzodiazepine-like substances. The limits of detection (LOD) range from 0.0001 to 0.0126 mg/l. Linearity is satisfactory for all compounds. The extraction recoveries for the benzodiazepines in whole blood are between 60 and 91%, desmethyldiazepam, OH-bromazepam and brotizolam excepted. Selectivity, accuracy and precision are satisfactory for clinical and forensic purposes.
描述了一种在基质支持液-液萃取后使用高效液相色谱-质谱联用仪（液相色谱-质谱联用仪，离子阱）的定量方法，用于同时测定全血中33种苯二氮卓类药物，包括其代谢物和苯二氮卓类样物质。检测限（LOD）范围为0.0001至0.0126 mg/l。所有化合物的线性均令人满意。全血中苯二氮卓类药物的萃取回收率在60%至91%之间，去甲基安定、羟基溴西泮和溴替唑仑除外。对于临床和法医目的而言，选择性、准确度和精密度均令人满意。
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