State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Int J Mol Sci. 2013 Sep 25;14(10):19474-83. doi: 10.3390/ijms141019474.
It is challenging to detect 7-aminonitrazepam (7-ANZP) residue in animal tissues simply and sensitively by the enzyme-linked sorbent immunoassay (ELISA) method. This paper demonstrates that utilizing a bioconjugate of gold nanoparticles and enzyme-labeled antibody as a signal probe increases the sensitivity of a traditional ELISA for 7-ANZP by nearly 20 times. The sensitivity of this ELISA for 7-ANZP was 5.6 pg/mL in buffer, and the limit of detection (LOD) of 0.18 µg/kg for 7-ANZP in urine could be achieved after the urine samples were simply hydrolyzed and diluted by buffer. This simple and sensitive method has potential application for improving the sensitivity of ELISA methods against various small molecules.
用酶联吸附免疫法（ELISA）方法简单、灵敏地检测动物组织中的 7-氨基硝西泮（7-ANZP）残留物具有挑战性。本文证明，利用金纳米粒子与酶标记抗体的生物缀合物作为信号探针，可使传统的 7-ANZP ELISA 检测的灵敏度提高近 20 倍。该 ELISA 对 7-ANZP 的检测灵敏度在缓冲液中为 5.6 pg/mL，尿液中 7-ANZP 的检测限（LOD）为 0.18 µg/kg，尿液样品经简单水解和缓冲液稀释后即可达到。这种简单灵敏的方法可能有助于提高针对各种小分子的 ELISA 方法的灵敏度。
