固定化β-环糊精作为一种简单且可循环利用的去除牛奶中胆固醇的方法。

Immobilized beta-cyclodextrin as a simple and recyclable method for cholesterol removal in milk.

作者信息

Kwak H S, Kim S H, Kim J H, Choi H J, Kang J

机构信息

Department of Food Science and Technology, Sejong University, Seoul 143-747, Korea.

出版信息

Arch Pharm Res. 2004 Aug;27(8):873-7. doi: 10.1007/BF02980182.

DOI:10.1007/BF02980182

PMID:15460451

Abstract

This study was designed to determine the optimum conditions of three different factors (mixing time, mixing temperature, and tube size) in reduction of cholesterol in milk using immobilized beta-CD beads. Immobilized beta-CD glass beads were prepared at different conditions of silanization and beta-CD immobilization reactions. In result, the glass beads (diameter 1 mm) at 20 mM 3-isocyanatopropyltriethoxysilane and 30 mM beta-CD without base showed the highest cholesterol removal rate as 41%. Using above immobilized beta-CD glass beads, the cholesterol removal rate was 40.2% with 6 h of mixing time in 7 mm diameter tube at 10 degrees C. After cholesterol removal from milk, the glass beads were washed for cholesterol dissociation and reused. In recycling study, the cholesterol removal rate was 41%, which was mostly same as that using new glass beads. These results indicated that cholesterol removal rate was about 40% with beta-CD immobilized glass beads, however, the recycling efficiency was almost 100%.

摘要

本研究旨在确定使用固定化β-环糊精珠降低牛奶中胆固醇时三种不同因素（混合时间、混合温度和管径）的最佳条件。在不同的硅烷化和β-环糊精固定化反应条件下制备了固定化β-环糊精玻璃珠。结果表明，在20 mM 3-异氰酸丙基三乙氧基硅烷和30 mMβ-环糊精且无碱的条件下制备的玻璃珠（直径1 mm）胆固醇去除率最高，为41%。使用上述固定化β-环糊精玻璃珠，在10℃下于7 mm直径的管中混合6小时，胆固醇去除率为40.2%。从牛奶中去除胆固醇后，对玻璃珠进行洗涤以解离胆固醇并重复使用。在循环利用研究中，胆固醇去除率为41%，与使用新玻璃珠时基本相同。这些结果表明，使用固定化β-环糊精玻璃珠时胆固醇去除率约为40%，然而，循环利用效率几乎为100%。