Cheng Winton, Hsiao I-Shan, Chen Jiann-Chu
Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung, Taiwan, 912, ROC.
Dis Aquat Organ. 2004 Aug 9;60(2):157-64. doi: 10.3354/dao060157.
Taiwan abalones Haliotis diversicolor supertexta held in 30% per thousand seawater and 26 degrees C were injected with tryptic soy broth (TSB)-grown Vibrioparahaemolyticus (1.6 x 10(5) CFU [colony-forming units] abalone(-1)), and then placed in water containing different concentrations of nitrite-N (nitrite as nitrogen): 0.01 mg l(-1) (control), 1.05, 3.04, 5.10 and 10.06 mg l(-1). Mortality of the abalones increased in direct parallel to ambient nitrite-N concentration. Over 12 to 48 h, the mortality of V. parahaemolyticus-injected abalones held in 3.04 mg l(-1) nitrite-N was significantly higher than that of abalones in the control solution. Abalones that had been exposed to control, 0.96, 2.95, 5.03 and 10.16 mg l(-1) nitrite-N for 24, 72 and 120 h were examined for THC (total hemocyte count), phenoloxidase activity, respiratory bursts (release of superoxide anion), phagocytic activity, and clearance efficiency of V. parahaemolyticus. The THC increased in abalone after 72 h exposure to 0.96 and 2.95 mg l(-1) nitrite-N, but decreased in abalones after 24 h exposure to 5.03 and 10.16 mg l(-1) nitrite-N. Phenoloxidase activity and respiratory bursts increased, while phagocytic activity and clearance efficiency decreased in abalones exposed to > or = 0.96 mg l(-1) nitrite-N for 24 h. It is concluded that nitrite-N in water at concentrations as low as 0.96 mg l(-1) weakens the immune response and increases mortality of H. diversicolor supertexta infected with V. parahaemolyticus.
将台湾鲍鱼九孔鲍置于盐度为30‰的海水中，温度为26摄氏度，向其注射在胰蛋白胨大豆肉汤（TSB）中培养的副溶血性弧菌（每只鲍鱼1.6×10⁵ 菌落形成单位[CFU]），然后将其置于含有不同浓度亚硝酸盐氮（以氮计的亚硝酸盐）的水中：0.01毫克/升（对照）、1.05、3.04、5.10和10.06毫克/升。鲍鱼的死亡率与环境中亚硝酸盐氮浓度直接呈平行增加。在12至48小时内，置于3.04毫克/升亚硝酸盐氮水中的注射了副溶血性弧菌的鲍鱼死亡率显著高于对照溶液中的鲍鱼。对暴露于对照、0.96、2.95、5.03和10.16毫克/升亚硝酸盐氮24、72和120小时的鲍鱼进行了总血细胞计数（THC）、酚氧化酶活性、呼吸爆发（超氧阴离子释放）、吞噬活性以及副溶血性弧菌清除效率的检测。暴露于0.96和2.95毫克/升亚硝酸盐氮72小时后鲍鱼的总血细胞计数增加，但暴露于5.03和10.16毫克/升亚硝酸盐氮24小时后鲍鱼的总血细胞计数减少。暴露于≥0.96毫克/升亚硝酸盐氮24小时的鲍鱼，其酚氧化酶活性和呼吸爆发增加，而吞噬活性和清除效率降低。得出的结论是，水中低至0.96毫克/升浓度的亚硝酸盐氮会削弱九孔鲍的免疫反应并增加感染副溶血性弧菌的九孔鲍的死亡率。
